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| 施万细胞高效体外传代培养的实验研究 | |
| 引用本文： | 迟建光,郝伟,姜明,王福利,刘建平,董青川.施万细胞高效体外传代培养的实验研究[J].临床军医杂志,2009,37(6):946-948,F0004. |
| 作者姓名： | 迟建光 郝伟 姜明 王福利 刘建平 董青川 |
| 作者单位： | 1. 山东莱州市中医院,山东,莱州,261400 2. 解放军济南军区总医院,山东济南,250031 3. 解放军第四军医大学西京医院,陕西,西安,710032 |
| 摘 要： | 目的探讨获取大量高纯度施万细胞(SC)的体外培养纯化方法。方法取6~7 d SD乳鼠坐骨神经,立体显微镜下机械性剥除神经外膜与束膜,采用低浓度胰酶消化法和差速贴壁法去除成纤维细胞,阿糖胞苷与G enetic in联合应用进行SC纯化,牛脑垂体提取物(BPE)促进SC增殖。结果经形态学及S-100蛋白相关抗原免疫细胞化学染色鉴定证实所获得的SC状态良好,纯度较高,可达96%以上。结论本方法可以体外获取大量、高纯度的SC,为神经组织工程的研究奠定了实验基础。 |
| 关 键 词： | 施万细胞 体外培养 纯化 |
Experimental Study on Efficient Serial Subcultivation of Schwann Cells in Vitro |
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| Chi Jian-guang,Hao Wei,Juang Ming,Wang Fu-li,Liu Jian-ping,Dong Qing-chuan.Experimental Study on Efficient Serial Subcultivation of Schwann Cells in Vitro[J].Clinical Journal of Medical Officer,2009,37(6):946-948,F0004. | |
| Authors: | Chi Jian-guang Hao Wei Juang Ming Wang Fu-li Liu Jian-ping Dong Qing-chuan |
| Institution: | Chi Jian-guang1,Hao Wei2,Juang Ming2,Wang Fu-li3,Liu Jian-ping3,Dong Qing-chuan3(1.Laizhou Hospital of Traditional Chinese Medicine,Laizhou 261400,China,2.General Hospital of Jinan Military Area Command,Jinan 250031,3.Xijing Hospital of The Fourth Military Medical University,Xi'an 710032,China) |
| Abstract: | Objective To obtain an efficient method for culturing highly purified and large amount of Schwann cells(SCs) in vitro.Methods The sciatic nerves were removed aseptically under anatomical microscope from 6 to 7 days old Sprague-Dawley rats.Low concentration enzymatic dissociation and differential adhesion methods were used to eliminate fibroblasts.SCs were purified by Cytosine arabinoside and geneticin and proliferated by bovine pituitary extract(BPE).Results SCs were identified by SABC immunohistochemistry ... |
| Keywords: | schwann cell culture in vitro purification |
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