侧颅底冠状位组织学与CT断层的对照

目的 制作颞骨火棉胶冠状位断层连续薄层切片,获取数字化图片库。方法 用2例尸头标本进行螺旋CT扫描后制作断层标本火棉胶包埋后，用大型轮式切片机沿冠状位切片，厚度100μm，每片均摄影并保存于计算机。选取代表性层面的胶片进行HE染色。将断层照片与HE染色照
片和CT片进行对照观察，对代表性层面结构特点进行描述。结果 共获取侧颅底数字化连续冠状位薄层断面标本数据集 2套。断层图像分辨率1920×2560像素，结构毗邻关系显示清晰。与HE照片和CT片进行对照观察，可以更清楚地定位断层结构。结论 火棉胶包埋技术是制作颞骨大切
片，获取高分辨率的数字化图片数据集的理想方法。冠状位对听小骨、Prussak‘s间隙、颈静脉窝、耳蜗与中颅窝的关系，颈内动脉升段及膝部、面神经垂直段、内听道与中颅窝的关系等显示较佳。

Comparison of observations between coronal histological and CT images of the lateral skull base

Objective To obtain digital image data from sequential sectional coronary celloidin slices of the temporal bone in order to provide references for imaging sectional study and clinical diagnosis of the lateral skull base. Methods Two cadaveric
heads were used to make sectional specimens after spiral CT scanning. After celloidin embedding, a brain slicer was used for making thin slices(100 μm). Digital camera was used to take pictures after each slicing.Representative slices were stained by
HE method.Digital pictures,HE pictures and CT images were examined and comparied to identify characteristics of sectional structures. Results Two sets of sequential digital images were obtained with a resolution of 1920×2560 and spatial relationship among different
structures was clearly shown. Sectional structures were demonstrated more clearly after contrasting observation. Conclusion Celloidin embedding is an ideal method for obtaining high resolution sequential digital imaging data of the lateral skull base.
Coronary images are suitable to show ossicles, Prussak’s space,jugular vein fossa,the relationship between cochlear and middle cranial fossa, the jugular and petrous segment of internal carotid artery,the vertical segment of facial nerve,the relationship
between internal acoustic canal and middle cranial fossa.