Wnt通路抑制剂XAV-939对牙髓干细胞成脂分化的影响

目的：通过体外实验探讨Wnt通路抑制剂XAV?939对牙髓干细胞增殖及成脂分化的影响。方法酶消化法培养牙髓干细胞，鉴定后采用CCK8试剂盒检测XAV?939对牙髓干细胞增殖的影响，油红O染色检测XAV?939对牙髓干细胞成脂分化的影响，qRT?PCR检测成脂分化过程中，XAV?939对Wnt通路相关基因糖原合成酶激酶3β（glycogen synthase kinase?3β, GSK3β）和β?catenin以及成脂分化相关基因过氧化物酶体增殖物激活受体γ2（peroxisome proliferator activated receptor?γ2, PPARγ2）和CCAAT增强子结合蛋白α（CCAAT/enhancer binding protein α, C/EBPα）的影响。结果牙髓干细胞成脂诱导14 d后，XAV?939上调GSK3β、PPARγ2和C/EBPα的表达，同时下调β?catenin的表达。结论 XAV?939可以通过抑制Wnt通路促进牙髓干细胞的成脂分化。

Wnt inhibitor XAV-939 promote the adipogenic differentiation of dental pulp stem cells

Objective To evaluate the effect of Wnt inhibitor XAV?939 on the proliferation and adipogenic differen?tiation of dental pulp stem cells (DPSCs). Methods The effect of XAV?939 on the proliferation of DPSCs was analyzed by cell counting kit?8(CCK?8). The effect of XAV?939 on the adipogenic differentiation of DPSCs was analyzed by oil red staining. Quantificational real?time polymerase chain reaction (qRT?PCR) was used to analyze the gene expression of glycogen synthase kinase?3β(GSK3β) andβ?catenin which were Wnt signaling marker genes, and peroxisome prolif?erator activated receptor?γ2 (PPARγ2) and CCAAT/enhancer binding proteinα(C/EBPα) which were adipogenic mark?er genes. Results After 14 days, XAV?939 up?regulates expression of GSK3β、PPARγ2 and C/EBPα, while down?reg?ulatesβ?catenin. Conclusion XAV?939 could promote the adipogenic differentiation of DPSCs by inhibiting Wnt sig?nalling pathway.