早期母婴分离对大鼠成年后抑郁易感性和海马GluR1表达的影响

目的：探讨早期母婴分离对大鼠成年后抑郁易感性和海马GluR1表达的影响。方法将Sprague Dawley孕鼠分娩的56只新生雄性幼鼠随机分为对照组、母婴分离（MS）组、慢性重复性束缚应激（CRR）组和母婴分离并慢性重复性束缚应激（ MS＋CRR）组。 MS组和MS＋CRR组幼鼠出生后第2～14天进行母婴分离应激3 h。鼠龄达7周（体重≈290 g）后，CRR组和MS＋CRR组大鼠连续10 d给予3 h慢性重复性束缚应激。之后，进行旷场、新环境进食抑制及强迫游泳实验。结果与对照组、MS组和CRR组比较，MS＋CRR组旷场总距离明显减少，新环境进食抑制实验进食潜伏期和强迫游泳不动时间均显著延长（均P＜0.05）；MS组海马GluR1的表达较对照组降低（P＜0.05），MS＋CRR组海马GluR1的表达较其他3组均显著降低（P＜0.05）。结论早期母婴分离可造成大鼠成年后抑郁易感性，且该易感性与海马GluR1的表达减少有关。

Effects of maternal separation on vulnerability to depression and the expression of GluR1 in adult rats

Objective To investigate the effect of maternal separation on vulnerability to depression and the ex-pression of GluR1 in adult rats .Methods Fifty-six newborn male SD rats were randomly assigned to four groups , con-trol, MS, CRR and MS+CRR groups.The rats in MS and MS+CRR groups received maternal separated for 3 h on Day 2 to Day 14 after birth.The rats in CRR and MS+CRR groups were subjected to three -hour chronic repeated restraint stress ( CRR) for 10 days at 6 weeks old ( weight were sub behavioral changes were recorded by open -field test ( OFT) , novelty suppressed feeding test ( NST) and forced swim test ( FST) .Western blot was used to measure the expression of GluR1 in hippocampus .Results Compared with the control , MS and CRR groups , the total distance of OFT in MS +CRR group was significantly reduced , with significant increases of the latency time of NSF and the immobility time of FST (P<0.05).The expression of GluR1 in hippocampus in MS group was down -regulatedcompared to control group (P<0.05).Compared with the other three groups , significant down-regulation in GluR1 in hippocampuswas revealed in MS+CRR group (P<0.05).Conclusion Maternal separation causes vulnerability to depression in rats at adult age .And the expression of GluR1 in hippocampus is involved .