Mechanism of delayed intracranial hypertension after cerebroventricular infusions in conscious rats |
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Authors: | Bret A Morrow Michelle R Holt Vesna P Starcevic Lanny C Keil Walter B Severs |
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Institution: | aDepartment of Pharmacology, College of Medicine, The Pennsylvania State University, Hershey, PA 17033 U.S.A. bNASA - Ames Research Center, Moffett Field, CA 94035 U.S.A. |
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Abstract: | Prior studies showed that cerebroventricular infusions of artificial cerebrospinal fluid, 8 μl/min for 10 min, followed by a 10 min rest and a 24 h infusion of 0.5 μl/min, raised cerebrospinal fluid pressure (CSFp) of conscious, unrestrained rats after about 2 h. Here, we report that the 10 min infusion alone evoked a delayed, prolonged rise in CSFp. Pressure during the infusion itself rose and recovered quickly, as is usually reported. Pressure/volume tests, used to calculate resistance to outflow (Ro) and compliance (C), revealed that infusions increasedRo and decreasedC, after a delay (P < 0.05). The rise in CSFp after infusion was blocked by pretreatment with acetazolamide + ouabain (P < 0.05), but the delayed changes in Ro andC were unaffected. We suggest that the 10 min infusion of a sterile, balanced salt solution has a primary effect that increasesRo; as CSF synthesis continues, C is exhausted and the delayed rise in CSFp ensues. This non-traumatic method of raising CSFp may be a useful method to study intracranial fluid dynamics. |
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Keywords: | Intracranial pressure Cerebrospinal fluid pressure Animal model Cerebrospinal fluid dynamics Acetazolamide Ouabain |
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