大黄素对体外人肺腺癌细胞增殖抑制作用的影响

目的探讨大黄素对人肺腺癌Anip973细胞(简称肺癌细胞)作用的机制。方法采用四甲基偶氮唑蓝(MTT)比色法和生长曲线测定比较不同浓度的大黄素在不同作用时间内对体外培养的肺癌细胞增殖的影响，同时应用流式细胞术检测细胞凋亡率，并对此进行电镜分析。结果在一定范围内，大黄素的浓度越大、作用时间越长对肿瘤细胞生长的抑制作用越强，凋亡率也越高，60μmol／L大黄素处理肺癌细胞72h后增殖抑制率达90％。结论大黄素可明显抑制肺癌细胞的增殖，其抑制作用具有时效和量效关系。

Inhibitory Effect of Emodin on Proliferation of Human Lung Adenocarcinoma Cells in vitro

OBJECTIVE: To investigate the anti-tumor mechanisms of emodin on human lung adenocarcinoma cell line Anip973 (LACC). METHODS: The influence of emodin of different concentration at different time on LACC proliferation were determined and compared using MTT colorimeric assay and cell growth curve assay. And the cell apoptotic rate was determined by flow cytometry and analyzed with electronic microscope. RESULTS: In a certain range, the higher concentration and longer acting time of emodin could induce the stronger inhibitory effect on tumor cell growth and the higher apoptotic rate. The proliferation inhibitory rate reached 90% after LACC being treated with emodin 60 micromol/L for 72h. CONCLUSION: Emodin can significantly inhibit proliferation of human LACC, showing dose-effect and time-effect relationship.