导数多波长数据线性回归法测定脑清片中氨基比林和咖啡因的含量

根据在不同波长下两组分百分导数吸收系数的差异,本文提出用导数分光光度法同时测定带有辅料或其它呈一次或二次曲线吸收的两个干扰组分的“导数多波长数据线性回归法”。以同时测定脑清片中的氨基比林和咖啡因为例,研究了方法的可靠性和测量的适宜条件。在296.3～274.9nm间,选14个波长测量一阶导数吸收度。结果表明:本法简便、快速、准确度和精密度均满意。可用于对复方制剂的分析。

APPLICATION OF THE DERIVATIVE LINEAR REGRESSION METHOD OF MULTIWAVELENGTH DATA TO THE SIMULTANEOUS DETERMINATION OF AMIDOPYRINE AND CAFFEINE IN COMPOUND AMIDOPYRINE AND CAFFEINE TABLETS

In this paper, a derivative linear regression method of multiwave length data is proposed. The method, based on the difference of derivative specific extinction coefficient between two-component at multiwavelenvth, can be applied to simultaneous spectrophotometric determination of two-interfering-component systems with interference of excipients or other absorptions which belong to primary or quadratic curve absorption. As an example, the availability as well as the suitable condition for simultaneous determination of amidopyrine and caffeine in compound amidopyrine and caffeine tablets have been studied. The measured 1st derivative absorptivity(dA/dλ) at 14 wavelengths were over the range of 296.3～274.9 nm and the data were treated with the suggested method. The results obtained are satisfactory both in recovery and in precision. This method is simple, rapid and can be used for the analysis of compound medicinal preparations.