An immunological focus assay for murine leukemia viruses on viable attached cell lines

An assay is described for the detection and isolation of murine leukemia virus (MuLV)-infected cells in viable monolayers. The procedure utilizes antisera or monoclonal antibodies which specifically bind cell surface viral antigens of infected cells. Bound antibodies are subsequently detected by binding with ¹²⁵I-labeled Staphylococcus aureus protein A followed by autoradiography of the tissue culture vessel. Focal areas of infection can be identified from the autoradiograph and infected cells can subsequently be isolated and subcultured as MuLV-producing cell lines. With appropriate antibodies the procedure should be useful for the direct isolation of minor components, including mutants, in complex virus mixtures.