Effect of catalin on radical production in aqueous solutions and cross-linking in deoxyribonucleoprotein complex |
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| Authors: | Polyanskii N B Binyukov V I Mil' E |
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| Institution: | (1) Laboratory of Physicochemical Regulation of Cell Metabolism, Laboratory of Physicochemical Methods of Study of Nucleic Acids, N. N. Semenov Institute of Chemical Physics, Russian Academy of Sciences, Moscow;(2) Group of Membrane Biochemistry A. N. Bakh Institute of Biochemistry, Russian Academy of Sciences, Moscow |
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| Abstract: | The effect of catalin on cross-linking in the DNA-protein system is studied after illumination with visible and UV light.
Catalin is shown to stimulate this process. The use of a hydroxylamine trap reveals that catalin (10–100 μM) induces the accumulation
of free radicals in aqueous solutions as soon as 10 minutes after irradiation. A considerable amount of O
2
-
is fixed in the medium during illumination of catalin aqueous solution. Superoxide dismutase added to the incubation medium
considerably reduced the number of recorded radicals.
Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 118, N
o
8, pp. 142–144, August, 1994
Presented by I. P. Ashmarin, Member of the Russian Academy of Medical Sciences |
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| Keywords: | catalin deoxyribonucleoprotein complex superoxide anion radical lens cataract |
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