| 马勃多糖CGP-Ⅱ抗S180肉瘤及体外抗氧化研究 |
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| 引用本文： | 武翠玲,万兵,李钰娜.马勃多糖CGP-Ⅱ抗S180肉瘤及体外抗氧化研究[J].长治医学院学报,2016(3):169-171. |
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| 作者姓名： | 武翠玲 万兵 李钰娜 |
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| 作者单位： | 1. 长治医学院生物化学教研室 046000;2. 长治医学院第二临床学院 |
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| 基金项目： | 山西省高等学校大学生创新创业训练项目(2015299)；长治医学院科研启动基金项目(QDZ201517) |
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| 摘 要： | 目的：探讨马勃酸性多糖CGP‐Ⅱ抗S180肉瘤的作用及体外抗氧化作用。方法：水提醇沉法制得马勃粗多糖，再经 HiTrap Capto DEAE阴离子交换层析和Sephacryl S‐300 HR凝胶柱层析进一步纯化得到马勃酸性多糖CGP‐Ⅱ。采用咔唑‐硫酸法测得糖醛酸含量，同时建立小鼠S180肉瘤移植模型，研究CGP‐Ⅱ的体内抑瘤效应。利用氧自由基吸收法（Oxygen Radical Absorption Capacity ，ORAC）、DPPH法评价其体外抗氧化能力。结果：CGP‐Ⅱ糖醛酸含量为17.2％，200 mg／（kg?d）剂量组多糖对S180肉瘤的抑瘤率为48.4％，CGP‐Ⅱ具有一定的DPPH 自由基清除能力和氧自由基吸收能力。结论：酸性多糖CG P‐Ⅱ具有一定的抗肿瘤活性及体外抗氧化作用，本研究为其进一步开发利用奠定了基础。
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| 关 键 词： | 马勃 酸性多糖 S180肉瘤 DPPH ORAC 抗氧化活性 |
Lsolation,Purification of Acidic Polysaccharide from Calvatia Gigantea and Its Anti-S180 Sarcoma Activity |
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| Abstract: | Objective :To study the anti‐S180 activity and antioxidant effects of acidic polysaccharide from Calvatia gigantea .Methods:Water extracting and ethanol precipitating methods were applied to extract polysaccharide from Calvatia gigantea .Mice tumor model was established with transplantion of sarcoma S 180 .In addition ,antioxidant activity of CGP‐Ⅱwas evaluated by DPPH free radical scavenging assay and oxygen radical absorbance capacity (ORAC) assay .Results :The content of uronic acid in CGP‐Ⅱ was 17.2% .The inhibition rates of CGP‐Ⅱ on sarcoma S180 in mice was 48.4% ,when the dosage of CGP‐Ⅱ reached to 200 mg/(kg ? d) .CGP‐Ⅱ showed antioxidant capacity against DPPH and oxygen radical absorption capacity .Conclusion:CGP‐Ⅱ showed obvious antitumor and antioxidant effects .This research will provide important basis for the further research and application of CGP‐Ⅱ . |
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| Keywords: | Calvatia gigantea acidic polysaccharide sarcoma S180 DPPH ORAC antioxidant activity |
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