水通道蛋白5基因对小鼠树突状细胞功能影响的研究

目的 探讨水通道蛋白5(AQP5)基因的表达对小鼠骨髓来源的树突状细胞(bonemarrow-derived dendritic cells,BMDC)分化成熟的影响.方法 分离培养AQP5基因敲除(AQP5-/-)的小鼠及野生型小鼠的BMDC,采用LPS诱导其成熟,采用FACS检测DC表型和内吞作用的变化,采用3H-TdR掺入法检测其对异种淋巴细胞的刺激能力.结果 与野生型小鼠相比,AQP5基因敲除后DC表面共刺激分子CD40、CD80、CD86的表达下降;其内吞能力下降;野生型小鼠来源的DC在与颗粒性蛋白质接触30 min后仍可继续上升,而AQP5-/- 来源的DC在相互作用30 min后内吞能力达到高峰;野生型小鼠来源的DC对异种淋巴细胞的刺激能力远大于AQP5-/-小鼠.结论 AQP5介导的跨膜水转运对于DC功能的正常发挥具有重要意义.其具体信号途径有待进一步研究.

Disfunction of the dendritic cells from the aquaporin 5 knockout mice

Objective To investigate the effect of aquaporin 5(AQP5)gene to the differentiation and development of bone marrow-deriued dendritic cells(BMDC).Methotis The DCs obtained from the bone marrow of the AQP5-/- and the wild type(WT)mice were cultured and induced to maturation by LPS.The phenotype of the two types DCs were assayed by FACS,and the phagocytosis ability Was assayed by co-incubating with FITC-conjucted ovalbumin.Results Contrast to the DCs from the WT mice,the DCs from the A9P5-/-mice showed reducing positive rate of expression of the co-stimulating molecular such as CD40,CD80.CD86.And the phagoeytosis ability of DCs from the AOP5-/-mice was lower than that of the WT mice.The relationship of the phagncytosis rate to the time from the AOP5-/- mice was different from that of the WT mice.The stimulating ability of the AOP5-/-DCs was lower than that of the WT mice.Conclusion Water transmembrane moving mediated by AQP5 iS very important to the normal function of DCs.The certain mechanism of the signal moleeular is to be determined. Aquaporin 5;Dendritic cell;Gene knockout;Co-stimulating molecular