结核性脑膜炎患者脑脊液基质金属蛋白酶-9及其抑制剂检测

目的:探讨结核性脑膜炎患者脑脊液中基质金属蛋白酶-9(MMP-9)和基质金属蛋白酶组织抑制剂1(TIMP-1)水平的变化意义。方法:选取5例非肿瘤非感染性头痛患者脑脊液作对照,9例病毒性脑膜炎,12例结核性脑膜炎患者。12例结核性脑膜炎根据有无意识障碍或脑实质损害的症状分为两组,A组有意识障碍或脑实质损害症状,7例;B组无意识障碍或脑实质损害症状,5例。酶联免疫吸附测定(ELISA)方法检测脑脊液MMP-9和TIMP-1水平。结果:结核性脑膜炎脑脊液MMP-9的水平及MMP-9/TIMP-1比例显著增高,A组增高得更显著。结论:脑脊液MMP-9水平及MMP-9/TIMP-1比例对结核性脑膜炎的诊断和预后有参考价值。

Assay of MMP-9 and TIMP-1 in CSF of patients with tuberculous meningitis

Objective To observe the changes of matrix metalloproteinase-9(MMP-9)and its tissue inhibitor TIMP-1 in cerebrospinal fluid(CSF)of patients with tuberculous meningitis.Methods An enzyme-linked immunosorbent assay(ELISA)was used to detect MMP-9 and TIMP-1 in the CSF of patients with tuberculous meningitis(n = 12),virus meningitis(n = 9)and the headache controlled patients(n = 5).The patients with tuberculous meningitis were divided into two groups according to the patients with(Group A,n = 7)or without(Group B,n = 5)central nervous system complications such as conscious disturbance or symptomes of cerebral parenchymatous damage.Rusults MMP-9 and MMP-9/TIMP-1 ratio in CSF were significantly higher in CSF of patients with tuberculous meningitis and even more higher in Group A.Conclusions It is suggested that tuberculous meningitis patients with conscious disturbance or symptomes of cerebral parenchymatous damage is associated with a significantly increased levels of MMP-9 in CSF.So,assay of MMP-9 in CSF may be useful to the diagnose and prognosis of tuberculous meningitides.