PCR－DNA指纹图识别异基因骨髓移植后重建造血的细胞起源

用多聚酶链反应（ＰＣＲ）扩增多态性小卫星ＤＮＡ的３３．１，３３．６位点，联合地高辛标记寡核苷酸探针杂交方法，以小卫星ＤＮＡ的ＰＣＲ－ＤＮＡ指纹图为特异性遗传标志，识别２例慢性粒细胞白血病患者接受同胞供髓的骨髓移植后造血重建的细胞起源。结果显示均有供者源性造血细胞植活。该方法灵敏度高，特异性强，无同位素污染，尤其适用于同性别、同血型、ＨＬＡ全相合的异基因骨髓移植植活指标的监测。

RECOGNIZING THE CELLULAR ORIGIN OFHEMATOLOGICAL RECONSTITUTION AFTERALLOGENEIC BONE MARROW TRANSPLANTATIONBY PCR-DNA FINGERPRINTING

e combined PCR amplification of minisatelliteDNA loci 33. 1 ,33. 6 with digoxingeninlabelled locusspecific oligonucleotide hybridization and applied PCR-DNA fingerprint of the minisatellite DNAs as a geneticmarker to recognize the cellular origin of hematologicalreconstitution after allogeneic bone marrow transplan-tation (allo-BMT)in two patients with CML. Thetwo patients received bone marrow each frorn their sibsof the sarne gender and hloodtype as that of them.The results showed that the peripheral blood cells inboth patients were of the donor origin. This techniqueis highly sensitive, specific and especially useful in documenting the origin of peripheral blood cells of theHLA-identical allo-BMT of the same gender and bloodtype.