| 肺鳞癌mtDNA D-环序列微卫星不稳的研究 |
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| 引用本文： | 刘丽红,胡义德,钱海洪,钱频,张国强.肺鳞癌mtDNA D-环序列微卫星不稳的研究[J].第三军医大学学报,2007,29(10):889-891. |
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| 作者姓名： | 刘丽红 胡义德 钱海洪 钱频 张国强 |
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| 作者单位： | 第三军医大学新桥医院全军肿瘤诊治中心,重庆市肿瘤生物技术研究所,重庆,400037 |
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| 摘 要： | 目的 分析肺鳞癌患者癌组织线粒体DNA(mitochondrial DNA, mtDNA)非编码D-环区序列的微卫星不稳(mitochondrial microsatellite instability, mtMSI)现象并探讨其意义.方法 应用改良一步法制备15例肺鳞癌患者癌组织mtDNA, PCR产物直接测序,对比分析D-环区序列mtMSI情况.结果 15例肺鳞癌患者癌组织mtDNA D-环区中,全部出现了mtMSI(100%),在3个不同位点上共发现30个mtMSI.结论 肺鳞癌患者mtMSI发生率高, D环区碱基序列的mtMSI可能与肿瘤的发生发展有关.
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| 关 键 词： | 肺鳞癌 线粒体DNA D-环区 微卫星不稳 |
| 文章编号： | 1000-5404(2007)10-0889-03 |
| 修稿时间： | 2006年1月16日 |
MSI mutation of mitochondrial DNA in lung squamous carcinoma |
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| LIU Li-hong,HU Yi-de,QIAN Hai-hong,QIAN Pin,ZHANG Guo-qiang.MSI mutation of mitochondrial DNA in lung squamous carcinoma[J].Acta Academiae Medicinae Militaris Tertiae,2007,29(10):889-891. |
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| Authors: | LIU Li-hong HU Yi-de QIAN Hai-hong QIAN Pin ZHANG Guo-qiang |
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| Abstract: | Objective To detect the microsatellite instability (MSI) of mitochondrial DNA (mtDNA) in lung squamous carcinoma and to investigate its significance in carcinogenesis. Methods Carcinoma tissues were obtained from 15 cases of lung squamous carcinoma patients and mtDNA was extracted by one-step method. D-loop fragments were amplified by PCR. mtMSI were determined by DNA sequencing. Results The mtMSI as detected in all of 15 lung squamous carcinoma patients. Thirty mtMSIs were found in 3 different nucleotide regions. Conclusion These results indicate that the frequency of mtMSI in lung squamous carcinoma patients is very high and mtMSI in D-Loop might play an important role in lung carcinogenesis. |
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| Keywords: | lung squamous carcinoma mitochondrial DNA D-loop region microsatellite instability |
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