HPLC法测定不同产地西洋参中人参皂苷Rg_1、人参皂苷Re、人参皂苷Rb_1含量

目的：对不同产地西洋参中人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1三种主要药效成分进行高效液相色谱的含量测定。方法：采用C18柱,以乙腈-0.1%磷酸溶液为流动相进行梯度洗脱,流速为1.1 ml/min,检测波长为203 nm,柱温为30℃。结果：人参皂苷Rg1、Re、Rb1、在测定范围内有良好的线性关系,其平均回收率为97.2%～99.0%,RSD为1.61%～2.03%。结论：该方法准确可靠,重现性好,应用性强。

Determination of ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1 from different regions by HPLC

Objective：To detect ginsenoside Rg1,ginsenoside Re,ginsenoside Rb1 of three active principles in American ginseng from different regions by HPLC.Methods：A C18 column was used by gradient elution of acetonitrile-0.1% H3PO4 solution as the mobile phase,the flow rate was 1.1 ml/min and the detective wavelength was set at 203 nm,the column temperature was set at 30℃.Results：All of the three compounds showed good linearities and recoveries were in the range of 97.2%-99.0% with RSD of 1.61%-2.03%.Conclusion：The method is accurate and suitable for the determination of ginsenoside Rg1,ginsenoside Re,ginsenoside Rb1.