| 高效液相色谱法测定鱼肝油乳剂中维生素A的含量 |
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| 引用本文： | 胡少卿. 高效液相色谱法测定鱼肝油乳剂中维生素A的含量[J]. 中国医药导报, 2011, 8(20): 101-102 |
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| 作者姓名： | 胡少卿 |
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| 作者单位： | 广东省人民医院药学部,广东广州,510080 |
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| 摘 要： | 目的：建立高效液相色谱法测定复合鱼肝油制剂中维生素A含量的方法。方法：采用高效液相色谱法,选择迪马（Diamonsil）C18（4.6 mm×250 mm,5μm）色谱柱,流动相为：甲醇-水（95∶5）,流速1.0 ml/min,检测波长为326 nm,柱温为35℃。结果：维生素A进样量在2.907 0～34.883 7 IU/ml范围内呈良好的线性关系（r=0.999 6）,平均回收率为98.84%。三个批次的复方鱼肝油制剂的含量分别为62.082、62.163、62.558 mg/瓶。结论：以高效液相色谱法检测复合鱼肝油制剂中维生素A的含量,结果准确、重现性好,精密度高,可作为该制剂的质量控制方法。
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| 关 键 词： | 维生素A 鱼肝油 高效液相色谱法 |
Determination of Vitamin A in Compound Morrhuate Preparation by HPLC |
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| HU Shaoqing. Determination of Vitamin A in Compound Morrhuate Preparation by HPLC[J]. China Medical Herald, 2011, 8(20): 101-102 |
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| Authors: | HU Shaoqing |
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| Affiliation: | HU Shaoqing Department of Pharmaceutical,the People's Hospital of Guangdong Province,Guangzhou 510080,China |
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| Abstract: | Objective：To develop an HPLC method for the determination of Vitamin A in Compound Morrhuate Preparation.Methods：The method was performed on Diamonsil C18（4.6 mm×250 mm,5 μm） column.The mobile phase consisted of methanol-water（95∶5）,at a flow rate of 1.0 ml/min.The UV detection wavelength was 326 nm and the injection volunm was 20 μl.The column temperature was 35℃.Results：The linear range of reserpine was 2.907 0-34.883 7 IU/ml（r=0.999 6）,with average recovery of 98.84%.The average contents of the three batches Compound Morrhuate Preparation were 62.082,62.163,62.558 mg/bottle respectively.Conclusion：Determination of Vitamin A in Compound Morrhuate Preparation by HPLC is rapid,accurate and suitable for the quality control of Compound Morrhuate Preparation. |
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| Keywords: | Vitamin A Compound Morrhuate Preparation HPLC |
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