| 表达大鼠脑源性神经营养因子腺相关病毒(rAAV-BDNF)对体外培养海马神经元保护作用的研究 |
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| 引用本文： | 张惊宇,赵节绪.表达大鼠脑源性神经营养因子腺相关病毒(rAAV-BDNF)对体外培养海马神经元保护作用的研究[J].中国免疫学杂志,2008,24(12). |
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| 作者姓名： | 张惊宇 赵节绪 |
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| 作者单位： | 吉林大学第一医院神经内科,长春,130021 |
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| 摘 要： | 目的：研究表达大鼠脑源性神经营养因子腺相关病毒（rAAV-BDNF）对体外培养海马神经元的保护机制。方法：通过去血清培养诱导神经元凋亡后转染rAAV-BDNF,通过DAPI、PI及Actin染色来统计rAAV-BDNF对血清饥饿引起的细胞凋亡的数量及形态的影响。结果：rAAV-BDNF病毒能够有效地抑制血清饥饿引起的细胞凋亡,其保护效率在65左右,并可以有效地保护血清饥饿引起的细胞形态损伤。结论：rAAV-BDNF可通过抑制凋亡的途径保护体外培养的海马神经元。
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| 关 键 词： | rAAV-BDNF 细胞凋亡 基因治疗 |
Protective effects of adeno-associated viral gene transfer of BDNF on the hippocampal neurons |
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| ZHANG Jing-Yu,ZHAO Jie-Xu.Protective effects of adeno-associated viral gene transfer of BDNF on the hippocampal neurons[J].Chinese Journal of Immunology,2008,24(12). |
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| Authors: | ZHANG Jing-Yu ZHAO Jie-Xu |
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| Institution: | ZHANG Jing-Yu,ZHAO Jie-Xu.Department of Neurology,the First Hospital of Jilin University,Changchun 130021,China |
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| Abstract: | Objective:To investigate the effective mechanism for transfection of rat brain derived neurotrophic factor(BDNF) gene by recombinant adeno-associated virus in the cultured hippocampal neurons.Methods:rAAV-BDNF was injected into the serum free-induced neurons.DAPI,PI and Actin stain was performed to measure the apoptosis cells and morphogenesis of the cells.Results:After the gene transfer,the survival rate of the serum free-induced neurons was increased by 65%.Conclusion:The recombinant adeno-associated viru... |
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| Keywords: | rAAV-BDNF |
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