砷剂对肺腺癌细胞凋亡及LRP、C—myc基因表达的影响 |
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引用本文: | 韩冰,石玉枝,霍建民. 砷剂对肺腺癌细胞凋亡及LRP、C—myc基因表达的影响[J]. 陕西肿瘤医学, 2009, 17(11): 2048-2050 |
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作者姓名: | 韩冰 石玉枝 霍建民 |
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作者单位: | 哈尔滨医科大学附属第一医院呼吸内科,黑龙江哈尔滨150001 |
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摘 要: | 目的:研究三氧化二砷(arsenictrioxide,As2O3)对人肺腺癌的抑制和诱导凋亡作用及对肺耐药蛋白基因(1ungresistance protein,LRP)、C—myc基因表达的影响及可能机制。方法:选用人肺腺癌A549细胞株,运用体外细胞培养法,MTF法,流式细胞术检测As2O3,对人肺腺癌的抑制和诱导凋亡作用;用反转录-聚合酶链反应(RT—PCR)方法检测LRP、C—mycmRNA的表达。结果:As2O3对人肺腺癌A549细胞具有抑制作用,其抑制率呈时间-剂量依赖关系。不同浓度的As2O3均可诱导凋亡。1.0μmol/L、2.0μmol/L的As2O3可下调LRP的表达。结论:As2O3具有抑制肿瘤细胞增殖作用,主要是通过诱导细胞凋亡实现的,其机制与下调LRP、C—myc表达有密切关系。
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关 键 词: | 三氧化二砷 肺腺癌 肺耐药蛋白 凋亡 |
Effect and mechanism of arsenic trioxide on LRP C - myc expression in human lung ade- nocarcinoma cells in vitro |
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Affiliation: | HAN Bing, SHI Yu - zhi, HUO Jian - min( Respiration Department of the First Hospital Affiliated to Harbin Medical University,Harbin 150001, China.) |
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Abstract: | Objective : To study the apoptosis and mechanism of arsenic tripxide ( As2O3 ) on human lung adeno- carcinoma cells. Methods: MTT FCM and RT - PCR tests were used to investigate apoptosis levels induced by arsenic trioxide and the effect on LRP and C - myc expression. Results : 1.0μmol/L and 2.0μmol/L arsenic trioxide can decrease LRP expression. Conclusion : Low concentration of As2 O3 may improve apoptosis of lung cancer by decreasing LRP expression. |
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Keywords: | arsenic trioxide lung cancer apoptosis LRP |
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