| (His)6-Arg-Arg-人胰岛素原在大肠杆菌中的高效表达 |
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| 引用本文： | 李晓红,余蓉,曾蓉,彭先凤,杨继虞.(His)6-Arg-Arg-人胰岛素原在大肠杆菌中的高效表达[J].药物生物技术,2003,10(3):144-148. |
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| 作者姓名： | 李晓红 余蓉 曾蓉 彭先凤 杨继虞 |
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| 作者单位： | 四川大学华西药学院生物制药教研室,成都,610041 |
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| 摘 要： | 为了提高胰岛素原的表达量，用pQE-40质粒构建了(His)6-Arg-Arg-人胰岛素原(His)6-Arg-Arg- human Proinsulin，RRhPI]的大肠杆菌表达系统。通过培养条件的优化，在摇瓶培养的条件下，目标产物：RRhPI以包涵体形式获得了高效表达，每升培养基收获湿菌体约27g，包涵体6g(干重1．8g)，RRhPI约540mg。该水平已超过现有文献报道的摇瓶培养的最高水平。
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| 关 键 词： | 人胰岛素原 大肠杆菌 表达 |
Enhanced Expression of (His) 6-Arg-Arg-human Proinsulin in Escherichia coli |
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| LI Xiao-hong,YU Rong,ZENG Rong,PENG Xian-feng,YANG Ji-yu.Enhanced Expression of (His) 6-Arg-Arg-human Proinsulin in Escherichia coli[J].Pharmaceutical Biotechnology,2003,10(3):144-148. |
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| Authors: | LI Xiao-hong YU Rong ZENG Rong PENG Xian-feng YANG Ji-yu |
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| Abstract: | To enhance the expression level of human proinsulin, Escherichia coli (E.coli) M15 harbouring a plasmid (His) 6 Arg Arg human proinsulin (RRhPI)/pQE 40 for human proinsulin production was cultured using flask. As a result, high expression level of the gene product, which accumulated as inclusion bodies, was obtained. 27g wet E.coli cells were harvested from 1L culture medium, and 6g inclusion body also. The harvest of our designed gene product RRhPI was about 540mg/L culture medium. |
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| Keywords: | Human proinsulin Escherichia coli Expression |
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