原位聚合酶链反应检测结核分枝杆菌L型的初步研究

目的：建立一种不需提取ＤＮＡ的检测分枝结核杆菌Ｌ型的分子生物学方法。方法：采用原位聚合酶链反应（原位ＰＣＲ）技术，扩增结核杆菌Ｌ型及其感染的人和小鼠血标本中的结核杆菌ＩＳ６１１０基因，再用免疫组化技术显色。结果：结核杆菌Ｌ型菌株中的ＤＮＡ被扩增，血标本中，可见到红细胞和淋巴细胞周围粘附大量结核杆菌ＤＮＡ阳性颗粒。结论；红细胞和淋巴细胞具有免疫粘附功能，原位ＰＣＲ快速、简便，可用于检测稳定和不稳定分

Detection of Mycobacterium tuberculosis L-forms by in situ polymerase chain reaction

Objective:To establish a molecular biological method for detection of Mycobacterium tuberculosis (Mtb) L forms without DNA extraction.Methods: In situ PCR technique was used for amplification of Mtb IS 6110 gene in mycobacteria L forms strain and in blood specimens from patients or mice,and immunohistochemical ABC method was used for expression.Results:Amplified DNA was seen in the bacterial cells and a large number of DNA granules of Mtb clustered around lymphocytes and red blood cells.Conclusions:Red blood cells and lymphocytes have immune adherence functions, in situ PCR is a rapid,simple method of detecting stable and unstable mycobacteria L forms. [