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A method for transforming human liver epithelial cells by transfection using a plasmid containing SV40 early region gene |
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| Authors: | T. Tokiwa M. M. Lipsky D. T. Smoot Dr. J. F. Lechner |
| Affiliation: | (1) Laboratory of Hepatitis Research, Division of Virology, Food and Drug Administration, Bethesda, MD, USA;(2) Department of Pathology, University of Maryland, Baltimore, MD, USA;(3) Department of Medicine, Howard University College of Medicine, Washington, DC, USA;(4) Inhalation Toxicology Research Institute, 87185 Albuquerque, NM, USA;(5) Present address: Bionic Design Research Group, National Institute for Advanced Interdisciplinary Research, AIST/MITI, Higashi, Tsukuba, Ibaraki, Japan |
| Abstract: | Summary A method is described for establishing continuous cell lines of liver epithelial cells by transformation of cultured hepatocytes isolated by collagenase/dispase of adult human liver tissue. Between 2 to 5×105 hepatocytes are inoculated into a 60 mm culture dish. The cells are incubated in a serum-free medium. Once the cells begin to divide, they are transformed by transfection with a plasmid containing SV40 early region genes. |
| Keywords: | Human hepatocytes SV40 T antigen gene Transfection Transformed epithelial cell line |
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