口服防治血吸虫病新药的初筛方法

本文对新型防治日本血吸虫病药物的初篩方法进行了初步探讨。预防或治疗日本血吸虫病新药的筛选剂量,可以得自6鼠3天测毒的结果。小白鼠感染尾蚴当日起连续服药14天,停药次日解剖,用冲洗法冲出肝内童虫,测量其平均长度。如对照组平均长度与服药组相比的比率大于2,而且部分童虫的长度不及300微米,可认为该药有抑制童虫的作用。小白鼠感染尾蚴后4周,连续服药5—7天,停药次日解剖。如体虫雌虫缩短,色素明显减退,则认为该药对成虫有作用。

SCREENING METHOD FOR ORAL ANTIBILHARZIAL DRUGS IN MICE

The present report deals with a preliminary screening method for new drugs in prophylactic or therapeutic treatment of experimental schistosomiasis japonica. The dosage selected in the screening was calculated according to the result of a 3day toxicity test of 6 mice. In the preliminary prophylactic screening test, mice were treated orally from the day of infection for 14 days and were autopsied at the end of treatment. The schistosomula in the liver were collected by perfusion method and their lengths were measured. The drug may be considered effective in suppressing the growth of schistosomula if the mean length of the worms in the control group is twice that of the test group and a certain proportion of the schistosomula in the treated group is less than 300 micron in length. In a preliminary therapeutic screening test mice were treated one month following exposure to cercariae for 5—7 days, Mice were killed the day after withdrawal of the drug. The flukes were washed out and examined for changes in size and intestinal pigment content. The drug is considered to be active if the worms decreased in size and in their pigment content.