乙型肝炎表面抗原定量与原发性肝癌的相关性研究

目的：比较慢性乙型肝炎( CHB)与HBV相关性原发性肝癌患者中乙肝表面抗原( HBsAg)和HBV DNA载量情况。方法采用化学发光法检测403例CHB及肝细胞肝癌( HCC)患者血清乙型肝炎病毒标志物滴度,采用荧光定量PCR技术检测患者血清HBV DNA载量。403例患者按照临床诊断分为CHB组(209例)和HCC组(194例)。结果 CHB组：血清HBsAg 滴度≥250 IU/mL者占89．00%；HBV DNA≥1000 copies /mL者占88．40%。 HCC组：血清HBsAg滴度≥250 IU/mL者占79．90%；HBV DNA≥1000 copies /mL 者占67．70%。两组HBsAg≥250 IU/mL患者的比例差异、HBV DNA≥1000 copies/mL 患者的比例差异均有统计学意义(P<0．05)。结论与CHB相比,HBV相关性原发性肝癌患者HBsAg、HBV DNA载量较低。

Association between hepatitis B virus surface antigen quantification and HBV-related hepatocellular carcinoma

Objective To compare the HBsAg and HBV DNA load between chronic Hepatitis B ( CHB) and HBV-related hepatocel-lular carcinoma ( HCC) patients. Methods A total of 403 patients with chronic Hepatitis B ( CHB) and HCC were enrolled. HBV serologi-cal markers were tested by chemoluminescence method, and serum HBV-DNA level was detected by fluorescent quantitation PCR technology. All patients were divided into CHB group(209 patients) and HCC group(194 patients) according to clinical diagnosis. Results In CHB group, 89. 00% patients had serum HBsAg titers≥250 IU/mL and 88. 40% patients had HBV DNA≥1 000 copies/mL. In the meantime, 79. 90% and 67. 70% patients with HCC had serum HBsAg titers≥250 IU/mL and HBV DNA≥1 000 copies/mL, respectively. The differ-ences in HBsAg≥250 IU/mL, HBV DNA≥1 000 copies/mL between the two groups was significant (P<0. 05). Conclusion Compared with chronic hepatitis B, the patients of HBV-related hepatocellular carcinoma has lower HBsAg and HBV DNA load.