| BMP-2对室管膜前下区神经干细胞向多巴胺能神经元分化及对DLX5表达的影响 |
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| 引用本文： | 刘胜华,周政,杨辉,刘俊,阴金波,辛志成. BMP-2对室管膜前下区神经干细胞向多巴胺能神经元分化及对DLX5表达的影响[J]. 第三军医大学学报, 2009, 31(6) |
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| 作者姓名： | 刘胜华 周政 杨辉 刘俊 阴金波 辛志成 |
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| 作者单位： | 第三军医大学新桥医院神经外科,重庆,400037 |
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| 基金项目： | 全军医学科研计划面上项目 |
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| 摘 要： | 目的 探讨骨形成蛋白2(bone morphogenetic protein-2,BMP-2)对室管膜前下区(anterior subventricular zone,SVZa)神经干细胞(neural stem cells,NSCs)向多巴胺能神经元分化及对DLX5表达的影响.方法 体外培养SVZa NSCs,分为对照组、BMP-2组、BMP-2+Noggin组和Noggin组,通过免疫荧光染色和流式细胞术,观察其分化为酪氨酸羟化酶阳性(TH+)神经元的情况;用RT-PCR检测DIX5 mRNA表达水平.结果 BMP-2组SVZa NSCs向TH+神经元分化的比例和DLX5 mRNA表达水平均显著高于对照组(P<0.05),且这种效应能被其拮抗剂Noggin特异性抑制.结论 BMP-2可能通过上调DLX5表达水平从而促进SVZa NSCs向多巴胺能神经元分化.
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| 关 键 词： | 神经干细胞 室管膜前下区 多巴胺能神经元 |
Effects of BMP-2 on dopaminergic neuronal differentiation of neural stem cells from SVZa and on DLX5 expression |
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| LIU Sheng-hua,ZHOU Zheng,YANG Hui,LIU Jun,YIN Jin-bo,XIN Zhi-cheng. Effects of BMP-2 on dopaminergic neuronal differentiation of neural stem cells from SVZa and on DLX5 expression[J]. Acta Academiae Medicinae Militaris Tertiae, 2009, 31(6) |
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| Authors: | LIU Sheng-hua ZHOU Zheng YANG Hui LIU Jun YIN Jin-bo XIN Zhi-cheng |
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| Affiliation: | LIU Sheng-hua,ZHOU Zheng,YANG Hui,LIU Jun,YIN Jin-bo,XIN Zhi-cheng (Department of Neurosurgery,Xinqiao Hospital,Third Millitary Medical University,Chongqing 400037,China) |
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| Abstract: | Objective To investigate the effect of bone morphogenetic protein-2 (BMP-2) on dopaminergic neuronal differentiation of neural stem cells (NSCs) of anterior subventricular zone (SVZa) and expression of DLX5. Methods The neural stem cells from SVZa were separated and cultured in vitro, then divided into control group, BMP-2 group, BMP-2+Noggin group, Noggin group. TH+ neuronal differentiation of neural stem cells from SVZa was identified by immunofluorescence staining and flow cytometry. RT-PCR was employed ... |
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| Keywords: | DLX5 BMP-2 |
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