Vasostatin基因对胰腺癌细胞和血管内皮细胞增殖的影响

目的研究Vasostatin转基因对胰腺癌细胞、血管内皮细胞的作用,探讨其对胰腺癌生长抑制的作用机制。方法应用腺病毒载体将Vasostatin基因转入人胰腺癌细胞SW1990、人脐静脉血管内皮细胞ECV304,应用MTT方法检测转基因后细胞的生长活力。同时,应用小管形成实验研究Vasostatin对血管内皮细胞ECV304体外血管生成的影响。结果Vasostatin转基因对人胰腺癌SW1990细胞生长无显著影响。Vasostatin转基因(MOI分别为25和50)对人脐静脉血管内皮细胞ECV304作用72h后,Ad-Vasostatin组的ECV304细胞数目显著少于PBS组和Ad-lacZ组(P<0.05)。小管形成实验结果显示,Ad-Vasostatin组内皮细胞数目较少,细胞排列连续性差,可见条索状的细胞链,但中空闭合的管状结构缺如。结论腺病毒介导的Vasostatin基因可显著抑制人脐静脉血管内皮细胞ECV304的体外细胞增殖,抑制其体外血管生成,而对人胰腺癌肿瘤细胞SW1990的体外细胞增殖无明显影响。

Inhibitory effect of vasostatin on vascular endothelial cells.

Objective To investigate the effects of vasostatin on pancreatic cancer cells and vascular endothelial cells, and explore the mechanism of its inhibitory effect on xenogr afted pancreatic cancer in mice. Methods The effects of vasosta tin gene on the proliferations of SW1990 cells and ECV304 cells were measured by MTT assay. The effect of vasostatin on angiogenesis in vitro was evaluated by t ube formation assay. Results Cellular proliferation assay in vi tro did not reveal any different effect on SW1990 cells between Ad-Vasostatin g roup, Ad-lacZ group and PBS group ( P >0.05), while a significantly inhibitory effect was observed on ECV304 cells i n Ad-Vasostatin group compared to Ad-lacZ group and PBS group ( P < 0.05). The tube formation assay revealed that the endothelial cell strips were present with fewer cells and without closed hollow tubes in Ad-Vasostatin group compared with Ad-lacZ group and PBS group. Conclusions The vasostatin gene mediated by adenovirus had no effect on the proliferation of pancreatic cancer SW1990 cells, but could inhibit the p roliferation of human umbilical vein endothelial cell ECV304 in vitro, and signi ficantly inhibit the angiogenesis in vitro.