珍珠层水溶性基质对兔骨髓基质干细胞BMP-2和Cbfα1基因表达的影响

目的从珍珠层水溶性基质（WSM）对兔骨髓基质干细胞分泌的骨形成蛋白-2和核心结合因子基因表达的影响入手．研究WSM产生成骨作用的机制和途径。方法培养新西兰大白兔的骨髓基质干细胞（BMSCs），并用低温下提取的珍珠层水溶性基质作用后．检测不同浓度WSM作用下碱性磷酸酶的活性，制定剂量．效应曲线，确定量效关系；采用一步RT-PCR方法检测不同浓度WSM诱导兔BMSCs后骨形成蛋白-2和核心结合因子的基因表达量并进行统计学分析。结果WSM可以增加兔BMSCs中碱性磷酸酶的活性，在150～200wg／ml浓度之间作用明显，碱性磷酸酶活性增加显著：WSM诱导兔BMSCs后骨形成蛋白的表达量明显增加，核心结合因子的表达量无明显变化。结论WSM通过增加骨形成蛋白-2的基因表达量诱导兔BMSCs向成骨细胞分化，与核心结合因子的作用关系不明显。

Effects of soluble matrix of nacre on bone morphogenetic protein-2 and Cbfa1 gene expressions in rabbit marrow mesenchymal stem cells

OBJECTIVE: To provide insights into the mechanisms and pathways of osteogensis by observing the effects of water-soluble matrix of nacre (WSM) on bone morphogenetic protein-2 (BMP-2) and Cbfa1 gene expressions in rabbit marrow mesenchymal stem cells (BMSCs). METHODS: New Zealand rabbit BMSCs cultured in vitro were stimulated with different concentrations of WSM extracted at low temperature, and the activity of AKP in the cells was evaluated with the dose-effect curve generated. BMP-2 and Cbfa1 gene expressions in rabbit BMSCs exposed to WSM were assayed with one-step RT-PCR. RESULTS: The activity of AKP in rabbit BMSCs increased after stimulation with different concentrations of WSM, and the effects were the most obvious with the WSM concentration ranging from 150 to 200 microg/ml. BMP-2 gene expression in the BMSCs increased after WSM exposure, but which did not result in obvious changes in Cbfa1 gene expression. CONCLUSION: WSM induces differentiation of rabbit BMSCs towards osteoblasts by increasing BMP-2 gene expression, in which process Cbfa1 gene does not seem to play a significant role.