猪小肠黏膜下层作为盖髓剂的初步研究

目的 探讨猪小肠黏膜下层(small intestinal submucosa,SIS)的生物相容性及作为盖髓材料的可行性。方法 用Abraham法制备的猪小肠黏膜下层,与人牙髓细胞(human dental pulp cells,HDPCs)在体外复合培养并进行矿化诱导,观察细胞生长情况,并分别在复合培养第7、14、21天检测牙髓细胞的碱性磷酸酶活性水平,无材料的牙髓细胞矿化诱导组作为对照组。结果 牙髓细胞在猪小肠黏膜下层上生长良好,细胞表面有矿化晶体形成,说明其具有良好的生物相容性,其碱性磷酸酶活性水平在第7、14、21天均高于对照组。结论 Abraham方法制备的猪小肠黏膜下层有良好的生物相容性,在体外能促进牙髓细胞矿化,有望成为一种新型的盖髓剂。

Pilot study of using porcine small intestinal submucosa as pulp capping agent

Objective To investigate the biocompatibility of small intestinal submueosa and the feasibility of using SIS as pulp cap- ping agent. Methods SIS was prepared by the Abrahamg way. Human dental pulp cells （HDPCs） was co-cultured and induced min- eralization with SIS in vitro. The growth of HDPCs was investigated by dyeing and SEM. The level of alkaline phosphatase of HDPCs in co-culture with SIS and HDPCs which was cultured without SIS,was measured on the 7th day, 14th day and 21st day. Results HDPCs was growing well on the SIS, and setting out crystal. The level of alkaline phosphatase of HDPCs in co-culture was higher than that of the control group on the 7th day, 14th day and 21st day. The difference was significant. Conculusions The bioeompatibility of SIS, prepared by Abraham way, was nice. It was able to accelerate the mineralization of HDPCs in vitro. The using of SIS as pulp capping agent was feasible.