| 荧光定量PCR检测HBV DNA |
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| 引用本文： | 陈洪涛,贺春民,孙爱民,熊诗松. 荧光定量PCR检测HBV DNA[J]. 现代预防医学, 2003, 30(3): 326-327 |
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| 作者姓名： | 陈洪涛 贺春民 孙爱民 熊诗松 |
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| 作者单位： | 1. 河南省医学科学研究所肝病研究室,450052
2. 河南省正阳县卫生防疫站 |
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| 摘 要： | 目的：探讨血清HBV DNA水平与HBV标志物(HBV M)表现模式的关系。方法：对1207例慢性乙型肝炎患者进行血清HBV DNA荧光定量PCR分析，HBV M检测采用ELISA法。结果：血清HBV DNA水平与HBV M表现模式有关，HBsAg与HBeAg的存在影响HBV DNA水平变化。结论：HBbAg和HBV DNA有明显的相关，抗—HBe、抗HBc阳性者病毒末完全停止复制，只是复制水平降低。
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| 关 键 词： | 荧光定量PCR 检测 HBV DNA 慢性乙型肝炎 血清 |
| 文章编号： | 1003-8507(2003)03-0326-02 |
| 修稿时间： | 2002-10-09 |
NUCLEIC ACID QUANTIFYING ASSAY OF HEPATITIS B VIRUS AND ITS CLINICAL SIGNIFICANCE |
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| CHEN Hong-tao,HE Chun-min,SUN Ai-min,et al.. NUCLEIC ACID QUANTIFYING ASSAY OF HEPATITIS B VIRUS AND ITS CLINICAL SIGNIFICANCE[J]. Modern Preventive Medicine, 2003, 30(3): 326-327 |
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| Authors: | CHEN Hong-tao HE Chun-min SUN Ai-min et al. |
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| Affiliation: | CHEN Hong-tao,HE Chun-min,SUN Ai-min,et al.Henan Institute of Medical Sciences,Zhengzhou 450052. |
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| Abstract: | Objective:To explore the relation between the nucleic acid content of hepatitis B Virus and hepatitis B virus markers.Methods:1207 patients Suffering from Chronic hepatitis B were performed Amplisensor ration PCR analysis was used for HBV DNA quantifying assessment.Hepatitis B virus markers were detected by ELISA.Results:Serum HBV DNA content was related to hepaitis B virus markers.Conclusions:Thers is a Significant correlation between HBeAg and HBV DNA,but positive Anti-HBe and Anti-HBc have copies lower cover. |
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| Keywords: | Hepatitis B Virus HBVM HBVDNA Amplisensor |
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