乙型肝炎病毒多表位治疗性抗原基因的合成、表达及抗原性研究

目的 研究乙型肝炎病毒多表位治疗性疫苗基因的合成、表达及其产物的抗原性。方法 设计、并合成乙型肝炎病毒多表位抗原基因BPT，克隆人融合表达载体pWR450-1，构建重组质粒PWR／BPT。在大肠杆菌中表达乙型肝炎多表位抗原蛋白并纯化该蛋白，并用Westerm-blotting方法初步检测该抗原蛋白的抗原性。结果 成功构建了融合表达载体PWR／BPT，在大肠杆菌中表达出乙型肝炎多表位抗原蛋白，Westem-blotting检测显示该蛋白具有良好抗原性。结论 HBV多表位治疗性抗原基因的设计是成功的，其在大肠杆菌中表达的重组融合蛋白具有良好的抗原性，可能是较理想的HBV治疗性疫苗候选物。

Synthesis, cloning, expression and antigenicity of the therapeutic multi-epitope gene of hepatitis B virus and of its products]

OBJECTIVE: To study the synthesis, cloning, expression and antigenicity of therapeutic multi-epitope gene of hepatitis B virus. METHODS: The therapeutic multi-epitope gene of hepatitis B virus was synthesized and cloned into the vector pWR450-1, then was expressed in E.coli and the products were purified. The immunogenicity of the expressed protein was analyzed by Western-blotting. RESULTS: Recombinant plasmid PWR/BPT was constructed successfully and the protein of multi-epitope gene of hepatitis B virus was expressed in E. coli, which showed ideal antigenicity by Western-blotting. CONCLUSIONS: The designed multi-epitope therapeutic gene of hepatitis B virus was proved to be correct and the expressed protein may be a good therapeutic vaccine.