柴胡挥发油对大鼠肝能量代谢的影响

目的研究柴胡挥发油致大鼠肝毒性损伤的能量机制。方法大鼠ig给予柴胡挥发油0.19,0.28和0.42 m·lkg-1,连续15 d。Clark氧电极法测定肝线粒体呼吸耗氧量、呼吸控制率(RCR)和磷氧比值(P/O),测定ATP含量及定磷法测定ATP酶活性的变化。结果与正常对照组比较,柴胡挥发油0.42ml·kg-1可使大鼠肝线粒体和P/O从(1.76±0.28)%和1.28±0.38分别降至(0.76±0.12)%和0.71±0.21,呼吸耗氧量从(2.82±0.64)mol·min-1·g-1降至(1.04±0.23)mol·min-1·g-1,ATP含量从(1.00±0.18)μmol.g-1蛋白降至(0.72±0.13)μmol.g-1蛋白,Na+-K+-ATP酶、Mg2+-ATP酶和Ca2+-ATP酶活性分别从0.94±0.14,0.95±0.14和(0.97±0.16)mmol·h-1·g-1降至0.53±0.08,0.67±0.10和(0.65±0.11)mmol·h-1·g-1。柴胡挥发油0.28和0.19 ml·kg-1使大鼠肝脏线粒体PCR、P/O、呼吸耗氧量、ATP含量和ATP酶活性亦均显著降低(P<0.01)。结论柴胡挥发油可通过抑制线粒体呼吸功能、影响肝脏能量代谢造成肝毒性损伤。

Effect of volatile oil from Bupleurum chinese on energy metabolism of the liver in rats

OBJECTIVE To explore the energy mechanism of volatile oil from Bupleurum chinese for hepatotoxic injury. METHODS Volatile oil from B. chinese 0.19, 0.28 and 0.42 ml·kg^-1 was ig given to rats for 15 d continually. The changes in respiratory function, contents of ATP and acitivities of ATPases of hepatic mitochondrion were detected. RESULTS Compared with normal control group,the respiratory control rate (RCR) and phosphorus/oxygen rate(P/O) reduced from(1.76±0.28)% and 1.28±0.38 to (0.76±0.12)% and 0.71±0.21 in volatile oil from B. chinese 0.42 ml·kg^-1 group, respectively, and the respiratory oxygen uptake was reduced from (2.82±0.64) to (1.04±0.23)mol·min^-1·g^-1. Contents of ATP were reduced from (1.00±0.18) to (0.72±0.13)μmol·g^-1, and activities of Na⁺-K⁺-ATPase, Mg²⁺-ATPase and Ca²⁺-ATPase from 0.94±0.14, 0.95±0.14 and (0.97±0.16)mmol·h^-1·g^-1 protein to 0.53±0.08, 0.67±0.10 and (0.65±0.11)mmol·h^-1·g^-1 protein, respectively. Compared with normal control group, RCR, P/O, respiratory oxygen uptake, contents of ATP and ATPases in in volatile oil from B. chinese 0.28 and 0.19 ml·kg^-1 groups were also significantly reduced. CONCLUSION Volatile oil from B. chinese can cause hepatotoxicity in rats by inhibiting function of hepatic mitochondrion and influence the energy metabolism of the liver.