Coordinated expression of pro- and antiapoptotic proteins in the hippocampus of neonatal rats |
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Authors: | P N Men’shanov V V Muzyka N N Dygalo |
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Institution: | 1.Institute of Cytology and Genetics, Siberian Branch,Russian Academy of Sciences,Novosibirsk,Russia;2.Novosibirsk State University,Novosibirsk,Russia |
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Abstract: | We studied the contents of the apoptotic proteins Bcl-XL, Bax, and intact and active forms of caspase-3 using the immunoblot
method in the hippocampus and brainstem of 3-day-old rat pups. The expression of pro- and antiapoptotic proteins in the regions
of the developing brain demonstrates individual variability with a variation coefficient ranging from 10 to 40%. In the brainstem,
a structure with a low intensity of apoptosis at this period of ontogeny, correlations between the expression levels of these
proteins were not significant. However, in the hippocampus we observed a significant positive correlation between the levels
of expression of the proapoptotic proteins Bax and the active form of caspase-3 (r = +0.653). The levels of these proteins significantly negatively correlated with the content of the antiapoptotic protein
Bcl-XL (r = −0.651 and −0.740, respectively). Our data suggest coordinated expression of the apoptotic cascade proteins in the hippocampus,
a brain structure with a high intensity of elimination of excessive cells during early postnatal ontogeny. |
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