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| 高效液相色谱法测定狭叶红景天药材中红景天苷的含量 | |
| 引用本文： | 刘丹,崔颖,李灵芝,张丽.高效液相色谱法测定狭叶红景天药材中红景天苷的含量[J].武警医学院学报,2011,20(3):173-174,178. |
| 作者姓名： | 刘丹 崔颖 李灵芝 张丽 |
| 作者单位： | 1. 河北医科大学,河北,石家庄,050001;武警医学院药物化学与药物分析教研室,天津,300162 2. 武警医学院药物化学与药物分析教研室,天津,300162;天津市职业与环境危害生物标志物重点实验室,天津,300162 3. 武警医学院药物化学与药物分析教研室,天津,300162 |
| 基金项目： | 国家自然科学基金资助项目(30672774,81073152); 天津市科委重点项目(10JCZDJC21100) |
| 摘 要： | 【目的】建立测定红景天药材根茎中红景天苷含量的HPLC方法,为进一步研究红景天的药材质量提供依据。【方法】采用Thermo C18柱(250 mm×4.6 mm,5μm);乙腈-水-四氢呋喃(5.5:93:0.4)为流动相;流速为1.0 ml/min;紫外检测波长为270 nm;柱温30℃。【结果】红景天苷在2.5~40μg/ml范围内与峰面积呈良好的线性关系,回归方程为Y=1×107X-2428,r=0.9996。平均回收率为96.45%,RSD为0.63%(n=9)。【结论】本法灵敏,准确,稳定性与重现性均良好,可用于红景天药材中红景天苷的含量测定。 |
| 关 键 词： | 狭叶红景天药材 红景天苷 高效液相色谱法 |
Quantitative determination of salidroside in rhodiola kirilowii(Regel)Maxim.by HPLC |
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| LIU Dan,CUI Ying,LI Ling-zhi,ZHANG Li.Quantitative determination of salidroside in rhodiola kirilowii(Regel)Maxim.by HPLC[J].Acta Academiae Medicinae CPAPF,2011,20(3):173-174,178. | |
| Authors: | LIU Dan CUI Ying LI Ling-zhi ZHANG Li |
| Institution: | LIU Dan,CUI Ying,LI Ling-zhi,ZHANG Li(Medical College of Chinese people's Armed Police Force,Tianjin 300162,China) |
| Abstract: | 【Objective】To determine the content of salidroside in Rhodiola kirilowii（Regel） Maxim.by HPLC.【Methods】HPLC analysis was carried out on Thermo C18 column（250 mm×4.6 mm,5μm）.The mixture of acetonitrile-THF-water（5.5：93：0.4） was used as the mobile phase.The flow rate was 1.0 ml/min.The detection wavelength was at 270 nm.The column temperature was at 30 ℃.【Results】The linear range of salidroside was 2.5~40 μg/ml,the recovery of this method was 104.05% and the RSD was 0.54%（n =5）.【Conclusion】The method was sensitive,accurate and reproducible,and to suitable for the quality control of Rhodiola kirilowii（Regel） Maxim. |
| Keywords: | Rhodiola kirilowii(Regel) Maxim Salidroside High performance liquid chromatography |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |