蕨麻正丁醇部位对急性低压缺氧小鼠的保护作用

【目的】观察蕨麻正丁醇部位(n-butanol extract of Potentilla anserine L.,NP)对模拟高原缺氧损伤小鼠的保护作用。【方法】120只雄性昆明种小鼠随机分为正常对照组、低压缺氧模型组、红景天组和NP高0.3 g.(kg.d)-1]、中0.15 g.(kg.d)-1]、低剂量0.075 g.(kg.d)-1]组,每组20只。除正常对照组外,各组小鼠在密闭减压舱内,模拟海拔8 000 m维持缺氧18h。检测各组小鼠脑组织含水量,测定脑组织和血清超氧化物歧化酶(superoxide dismutase,SOD)活性和丙二醛(malondialdehyde,MDA)含量,血清肌酸激酶(creatine kinase,CK)活性。并通过HE染色观察各组小鼠脑组织缺氧损伤的程度。【结果】与减压缺氧模型组比较,红景天组和高剂量NP可显著降低减压缺氧小鼠脑含水量(P<0.01或P<0.05),提高小鼠脑组织SOD活力(P<0.01或P<0.05),减少小鼠脑组织MDA的产生(P<0.01或P<0.05),提高小鼠血清SOD活力(P<0.01),减少小鼠血清MDA的产生(P<0.01或P<0.05),降低小鼠血清中CK活性(P<0.01或P<0.05)。HE染色显示,NP及红景天胶囊可减轻小鼠低压缺氧对脑组织的损伤。【结论】蕨麻正丁醇提取部位对模拟高原缺氧损伤具有显著的保护作用。

N-butanol extract of Potentilla anserina L.inhibited injury resulted by acute hypobaric hypoxia in mice

【Objective】To study the protective effects of n-butanol extract of Potentilla anserina L.on acute hypobaric hypoxia in mice.【Methods】120 healthy male mice were randomly divided into six groups：normal control group,hypobaric hypoxia model group,Rhodiola Rosae jiaonang group0.6 mg·（kg·d）^-1],n-butanol extract of Potentilla anserina L.（NP） groups0.3 g·（kg·d）-1,0.15 g·（kg·d）^-1 and 0.075 g·（kg·d）^-1],（n=20）.All of the groups,except the control group,were underwent hypobaric hypoxia at atmosphere of-0.065 Mpa（equivalent to 8 000 m） in hypobaric chamber for 18 hours.The brain water content,the activity of SOD and the content of MDA in brain and serum were determined.The activity of CK in serum was also detected.The injury degree resulted from hypobaric hypoxia was observed by HE staining.【Results】Compared with hypobaric hypoxia model group,the SOD activity was increased and MDA content was decreased in brain and serum.Rhodiola Rosae Jiaonang and NP of each dose could significantly decrease the brain water content（P 〈0.01 or P 〈0.05）,increase the SOD activity and decrease the MDA content in brain and serum（P 〈0.01 or P 〈0.05）.Meanwhile they could increase the SOD activity in（P 〈0.01）,decrease the MDA content in serum（P 〈0.01 or P 〈0.05）,too.Moreover,decreased CK activity in serum was also observed in Rhodiola Rosae Jiaonang group and NP of each dose group of NP（P 〈0.01 or P 〈0.05）.HE staining showed that NP and Rhodiola Rosae could significantly attenuate the injury of brain induced by acute hypobaric hypoxia in mice.【Conclusion】NP showed a remarkable proective effect on acute hypobaric hypoxic injury in mice.