异氟烷对幼小鼠自主活动和学习记忆行为的影响

目的观察异氟烷(Iso)对幼小鼠自主活动和学习记忆行为的影响,探讨其与γ-氨基丁酸A受体(GABAA)的关系。方法 360只小鼠分为3大组,分别进行自主活动实验、避暗实验和跳台实验,每大组按分层随机区组设计分为正常对照组,Iso 0.05,0.1和0.2 ml·kg-1组、GABAA受体特异性阻断剂一叶萩碱(Sec)2,4和8 mg.kg-1组和Iso 0.2 m.lkg-1+Sec 2,4和8 mg.kg-1组。小鼠sc给予Sec 10 min后,再ip给予Iso,测试给药后15,30,45和60 min小鼠5 min内活动次数;跳台仪和避暗仪记录小鼠步入、跳下的潜伏期和错误次数。结果与同一时间点的正常对照组相比,Iso可减少小鼠自主活动次数,给予Iso后1 d小鼠避暗实验和跳台实验的步入和跳下潜伏期缩短以及错误次数增加(P<0.05)。正常小鼠单独给Sec(除Sec 8 mg·kg-1组15 min外)对小鼠自主活动、步入和跳下潜伏期和错误次数无明显影响,但Sec可改善Iso导致的小鼠自主活动次数,拮抗Iso对小鼠自主活动的影响,但随着时间延长拮抗作用逐渐减弱,明显低于正常对照组(P<0.05)。Sec延长Iso小鼠的跳下和步入潜伏期,减少错误次数,基本恢复至正常对照组水平,明显改善Iso对幼小鼠学习记忆的影响(P<0.05)。结论 Iso可降低幼小鼠自主活动次数,损害学习记忆能力,GABAA受体可能部分参与了以上作用。

Effects of isoflurane on spontaneous motor activity and learning behavior in juvenile mice

OBJECTIVE To investigate the effect of isoflurane(Iso) on spontaneous motor activity and learning behavior, and to explore their relationships with γ-aminobutyric acid A receptor (GABA_A) in juvenile mice. METHODS A total of 360 mice were divided into three groups for three experiments. Each group was subdivided into 10 subgroups: normal control, Iso 0.05, 0.1 and 0.2 ml·kg^-1, securinine(Sec) 2, 4 and 8 mg·kg^-1, and Iso 0.2 ml·kg^-1+Sec 2, 4 and 8 mg·kg^-1. Sec was sc given 10 min before Iso was ip given. The times of spontaneous motor activity in 15, 30, 45 and 60 min after the administration of Iso were recorded and the latency and error times of step down and step through tests at 1 d and 2 d were detected. RESULTS Compared with normal control group, Iso could decrease the times of spontaneous motor activity, and the latency but increase the error times of step down and step through tests in juvenile mice(P<0.05). Sec didn′t affect the times of spontaneous motor activity, the latency and the error times of step down and step through tests in juvenile mice. But Sec could decrease the effect of Iso on spontaneous motor activity, the latency and error times of step down and step through tests in juvenile mice(P<0.05). CONCLUSION Iso can decrease the times of spontaneous motor activity and restrain learning and memory functions in juvenile mice, and GABA_A receptor maybe involved in the effects.