我国不同流行周期脑膜炎奈瑟氏菌的亚型与流行病学意义

以11种抗脑膜炎奈瑟氏菌(Nm)1类外膜蛋白的单克隆抗体和全细胞酶联免疫吸附试验,对我国17省市于60年代至80年代,从流脑病人与带菌者中所搜集的193株A群Nm进行了亚型检定,并分析了其亚型与流脑周期流行的关系。分型结果表明,上述菌株至少可分成10个亚型,分型率为80.8％,其中P1.9亚型最多占39.9％,是我国60年代与80年代流脑流行时A群Nm的优势亚型,分别占两次流行时可分亚型菌株的100％和85.9％。其次为P1.7,10复合亚型,占29.5％,为70年代流行时的优势亚型,占可分亚型株数的74.3％。流行年A群Nm的优势亚型很突出,非流行年亚型分布则不集中,无明显的优势亚型。从病人及其密切接触者中可检出同一亚型。因此,A群Nm亚型的检定可以清楚地揭示流脑在我国周期流行的特征与其传播规律。

Subtypes of Neisseria Meningitidis Group A from Different Epidemie Cycles in China and Epidemiologic significances

193 strains of Neisseria meningitidis group A were isolated from the cases for epidemic meni- ngitis and the carriers during the 1960s-1980s in 17 provinces of China. Subtypes of the strains above mentioned and their epidemiologic significa- nces were investigated by 11 monoclonal antibodies against class 1 OMP of meningococci and whole -cell ELISA. It was found that these strains could be divided into 10 subtypes. Their subtypable rate was about 80.8%. The subtype P1.9 was the best common (39.9%) among the strains tested, And it was the predominant subtype of the cyclic preva ence and accounted for 100% and 85.9% of the subtypable isolated in 1960S and 1980S, respec- tively. The second common subtype was P1.7,1o mixed subtype and accounted for 29.5% of the strains tested. It was the predominant subtype of the epidemic cycles and accounted for 74.3% of the subtypable strains in 1970S. The predominant subtype was distributed widely in most of the provinces. Other subtypes were quite few and distributed in a few provinces of China. The predominant subtypes of Neisseria eningitidis group A were outstanding in epideic years. In contrast, the subtypes in nonepidec years were very scatter without obvious predominant subtyp- es. The identical subtypes between the strains of patients and their close contacts could be found. Therefore, the examination of subtypes of Neiss- eria meningitidis group A could clearly reveal the features of the cyclic prevalence and spreading law for epidemic cerebraspinal meningitis in China.