| 猪胰岛素与重组人胰岛素的高效液相色谱分析 |
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| 引用本文： | 孔毅,王伟刚,乔德水. 猪胰岛素与重组人胰岛素的高效液相色谱分析[J]. 中国生化药物杂志, 2003, 24(2): 84-85 |
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| 作者姓名： | 孔毅 王伟刚 乔德水 |
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| 作者单位： | 1. 南京大学生命科学学院,江苏南京210093
2. 江苏万邦生化医药股份有限公司,江苏徐州221002 |
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| 摘 要： | 目的鉴别猪胰岛素与重组人胰岛素。方法采用C8、C1 8及常规孔径 (6～ 1 0nm)与大孔径 (30nm)色谱柱用梯度洗脱和等度洗脱对猪胰岛素与重组人胰岛素的分离方法进行研究。结果用梯度洗脱代替英国药典中的洗脱体系能达到相同的分离效果。结论用常规C1 8柱通过梯度洗脱可分离猪胰岛素与重组人胰岛素
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| 关 键 词： | 猪胰岛素 重组人胰岛素 高效液相色谱法 |
| 文章编号： | 1005-1678(2003)02-0084-02 |
| 修稿时间： | 2002-07-10 |
Analysis of recombinant human insulin and porcine insulin by high-performance liquid chromatography |
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| KONG Yi ,WANG Wei gang ,QIAO De shui. Analysis of recombinant human insulin and porcine insulin by high-performance liquid chromatography[J]. Chinese Journal of Biochemical Pharmaceutics, 2003, 24(2): 84-85 |
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| Authors: | KONG Yi WANG Wei gang QIAO De shui |
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| Affiliation: | KONG Yi 1,WANG Wei gang 2,QIAO De shui 2 |
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| Abstract: | Purpose To identify recombinant human insulin and porcine insulin.MethodsC 8,C 18 ,big pore diameter(30 nm)and routine(6～10 nm) reversed phase high performance liquid chromatography(RP HPLC) column were used to analysis porcine insulin and recombinant human insulin by gradient and isocratic elution.ResultsThrough the optimization of column and analytical condition ,we can instead of complex buffer by simple one and also can make out better result.ConclusionThe routine(6～10nm) RP HPLC column is suitable to analyze porcine insulin and recombinant human insulin by gradient. |
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| Keywords: | recombinant human insulin porcine insulin HPLC |
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