乙型肝炎病毒C基因变屉对人白细胞抗原表达的影响

目的 阐明我国常见的ＨＢＶ／Ｃ变异对宿主细胞人类白细胞抗原（ｈｕｍａｎ ｌｅｕｋｏｃｙｔｅ ａｎｔｉｇｅｎ，ＨＬＡ）表达的影响。方法 构建携带野生型和变异型ＨＢＶ／Ｃ基因的真核细胞表达载体，转染ＨｅｐＧ２细胞，鉴定目的基因在宿主细胞中的表达，检测ＨＬＡ－１，ＨＬＡ－ＤＲ在宿主细胞膜上的表达。结果 ＰＣＲ和Ｗｃｓｔｅｒｎ ｂｌｏｔ分析能分别检测到目的ＤＮＡ片段和ＨＢｃＡｇ的表达，约１００％的ＨｅｐＧ

Influence of HBV/C mutation on HLA expression of host cells

Objective To study the influence of HBV/C mutation on host cellular HLA expression. Methods HBV expression vectors carrying wild or variant HBV/C gene were constructed and transferred into HepG2 cells. HBV/C gene expression on the host cells was identified. The expression of HLA-I and -DR on the host cell membrane was detected. Results DNA segments similar with HBV/C gene size and HBcAg were detected by PCR and Western blot analysis, respectively. Almost all HepG_2 cells expressed HLA--I but not HLA- DR. The fluorescence intensity of HLA- I expression on host cells was different: HepG_2 was 57.8 and wild vector 54.3. The wild HBV/C gene declined significantly to 31.2. While V60, G87 and L97 increased to 43.0, 54.0 and 69.4, respectively. L_97 was greatly increased with 11.6 higher than HepG2. HLA-DR had no significant expression. Conclusion Replication and expression of HBV/C gene can influence HLA-I expression on the host cells directly. Influence of variant gene on HLA-I expression is different from the wild gene. This might relate with the exacerbation of diseases resulting from HBV mutation.