氟伐他汀对大鼠心肌梗死后左室重构及caspase-3表达的影响

目的：观察氟伐他汀对大鼠心肌梗死后左室重构及凋亡基因caspase-3的影响。 方法： 复制心肌梗死动物模型，实验分为4组，Ⅰ组假手术组，Ⅱ组假手术+氟伐他汀组，Ⅲ组模型组，Ⅳ组模型+氟伐他汀组。Ⅱ和Ⅳ组术后给氟伐他汀10 mg·kg^-1·d^-1共4周，观察左室结构及心动超声变化，心肌羟脯氨酸含量及caspase-3表达。 结果： Ⅳ组心肌超微结构和左室重构指标改善程度明显高于Ⅲ组，羟脯氨酸含量及免疫组化caspase-3阳性细胞明显少于Ⅲ组（P<0.05），RT-PCR caspase-3 mRNA表达也明显少于Ⅲ组（P<0.05）。 结论： 氟伐他汀能改善大鼠心梗后左室重构，并下调凋亡基因caspase-3表达，抑制细胞凋亡。

Effect of fluvastatin on left ventricular remodeling and caspase-3 expression after myocardial infarction in rats

AIM: To observe the effect of fluvastatin (FV) on left ventricular remodeling and expression of caspase-3 after myocardial infarction (MI) in rats. METHODS: Rats were divided into 4 groups: group Ⅰ (sham), group Ⅱ (sham+FV), group Ⅲ (MI) and group Ⅳ (MI+FV). group Ⅱ and Ⅳ were treated with FV (10 mg·kg^-1·d^-1) for 4 weeks. The left ventricular structure, echocardiography and hydroxyproline were observed. The expression of caspase-3 was measured by immunohistochemistry and RT-PCR. RESULTS: Compared with MI group, there was a improvement of ultrastructure and index of left ventricular remodeling, and decrease in hydroxyproline in MI+FV group (all P<0.05). The number of caspase-3 positive cells also decreased in MI+FV group, and RT-PCR showed the level of caspase-3 mRNA expression was lower than that in MI group (P<0.05). CONCLUSION: Fluvastatin improves left ventricular remodeling after myocardial infarction, decreases the expression of caspase-3 and inhibits apoptosis.