葛根素对培养人脐静脉内皮细胞钙超载的影响

目的探讨葛根素对培养人脐静脉内皮细胞钙超载的影响。方法采用钙荧光探针F luo-3/AM标记培养的人脐静脉内皮细胞(HUVECs),激光共聚焦显微镜检测细胞胞内钙荧光信号,观察H2O2、ATP和高K+刺激作用下葛根素对培养的HUVECs中钙超载的影响。结果葛根素能抑制H2O2、ATP和高K+引起的[Ca2+]i升高,抑制率分别为72.2%、56.5%和78.2%;葛根素对高K+引起的钙超载的抑制作用与L-型电压依赖型钙通道的特异性拮抗剂Verapam il相似。结论葛根素可以缓解培养人脐静脉内皮细胞内钙超载,其作用机制可能与拮抗膜上电压依赖型钙通道和抑制内质网钙库释放有关。

Effect of Puerarin on calcium overload in cultured human umbilical vascular endothelial cells

Aim To examine the effect of Puerarin on calcium overload in human umbilical vascular endothelial cells(HUVECs).Methods The HUVECs were incubated with Puerarin,then were exposed to H_2O_2,ATP and high K~+.The changes of intracellular free calcium([Ca~(2+)]i) in HUVECs were determined using fluorescent Ca~(2+)-indicator fluo-3/AM with confocal laser scanning microscope.Results Puerarin inhibited the elevation of [Ca~(2+)]i induced by H_2O_2,ATP and high K~+,and the inhibition ratio were 72.2%,56.5% and 78.2% respectively.The effect of Puerarin inhibiting the elevation of [Ca~(2+)]i induced by high K~+ was similar to that of verapamil.Conclusion The mechanism of puerarin reducing the overload of calcium in HUVECs may be related with blocking both voltage-dependent calcium channels and the release of calcium pool in endoplasm.