HPV16/18E6和P53基因蛋白在喉鳞癌中的表达及其相关性研究

目的探讨人乳头瘤病毒16/18型早期蛋白E6(HPV16/18 E6)、P^53基因蛋白在喉鳞癌(LSCC)中的表达及其相关性.方法免疫组化SABC法检测HPV16/18E6和P^53基因蛋白在LSCC及声带息肉中的表达.结果 HPV16/18E6在声带息肉中未见表达,在LSCC中的表达率为40.0%,差异有显著性(P＜0.05). P^53基因蛋白在声带息肉与LSCC中的表达率分别为6.66%和66.2%,差异有显著性(P＜0.05).HPV16/18E6及 P^53基因蛋白的表达与LSCC临床分期、淋巴结转移有关.二者的表达无明显相关性.结论 HPV16/18型感染和P^53基因异常与LSCC发生、发展有关.HPV16/18E6与P^53基因功能异常无明显相关性.

Expression and correlation of HPV16/18E6 and P53 gene protein in laryngeal squamous cell carcinoma

Objective To study the expression of human papilloma virus 16/18E 6(HPV16/18E 6)and P 53 gene pvotein in laryngeal squamous cell carcinoma(LSCC) and the correla tion between them.Method Immuno- histochemical SABC technique was used to examine the expression of H PV16/18E 6 and P 53 gene protein in LSCC and polyp of vocal cord. Results In LSCC group,the positive rate of HPV16/18 E 6 w as 40.0%and the P 53 gene pvotein was 66.2%.In polyp of vocal cord gro up,the positive rate of P 53 gene pvotein was 6.66%and HPV16/18 E 6 was negative. There were significant association of expression of HPV16/18 E 6 and P 53 gene protein with clinic stage and lymph node metastases. The relationship betw een HPV16/18 E 6 andP 53 gene protein was not found.Conclu sion The infection of HPV16/18 and the abnormality of P 53 gene are closely related to the carcinogenesis and development of LSCC.There is no correlation between HPV16/18 E 6 and P 53 gene.