急性脊髓损伤患者血清蛋白质组学研究

目的观察急性脊髓损伤(SCI)患者和健康人群血清差异表达蛋白,寻找急性SCI相关特异性代谢通路或蛋白标志物。方法 2013年7月—2014年12月,采集9例急性颈部SCI患者(病例组)和9例年龄、性别与病例组相匹配的健康受试者(对照组)的血液样本。记录病例组损伤时间、颈椎日本骨科学会(JOA)评分等基本信息。应用非标记相对定量蛋白质组学技术比较病例组与对照组血清蛋白质谱,采用Spearman相关分析寻找差异表达蛋白与患者年龄、损伤时间以及JOA评分的相关性。应用基因本体论(GO)分析、BiNGO富集分析和京都基因与基因组百科全书(KEGG)对差异表达蛋白进行生物信息学分析。结果共发现22个差异表达蛋白。与对照组相比,病例组11个蛋白表达上调(包括果糖-二磷酸醛缩酶、碳酸酐酶等),且与损伤时间和颈椎JOA评分呈正相关;11个蛋白表达下调(包括免疫球蛋白等),且与损伤时间和颈椎JOA评分呈负相关。生物信息学分析发现这些差异表达蛋白主要富集在果糖和甘露糖代谢通路、血小板激活代谢通路、黏附连接代谢通路和氮代谢通路。结论急性SCI患者和健康人群血清蛋白质谱存在差异,果糖-二磷酸醛缩酶、碳酸酐酶等有望成为急性SCI潜在的分子标志物。

Serum proteomic analysis in patients with acute spinal cord injury

Objective To investigate the specific metabolic pathway or different biomarkers for acute spinal cord injury (SCI) by comparing the protein expression between acute SCI patients and healthy controls. Methods From July 2013 to December 2014, the blood samples were collected from 9 acute cervical SCI patients and 9 healthy controls. The demographic characteristics of age, time of injury, and cervical Japanese Orthopedic Association(JOA) score of the 9 SCI patients were recorded. The serum protein mass spectra were compared between SCI patients and healthy controls by label-free relatively quantitative proteomic technology. The correlation between differential protein expression and demographic characteristics was studied in the patients by using Spearman correlation analysis. The bioinformatic analyses of differential expression proteins were performed by using gene ontology(GO), BiNGO, and Kyoto encyclopedia of genes and genomes (KEGG) analyses. Results Twenty-two candidate proteins were identified in the acute SCI group, among which 11 proteins were up-regulated (such as fructose-bisphosphate aldolase, carbonic anhydrase) and positively correlated with the time of injury and JOA score; and 11 proteins (such as immunoglobulins) were down-regulated and negatively correlated with the time of injury and JOA score. Bioinformatic analysis showed the 22 proteins were mainly in fructose and mannose metabolism pathway, platelet activated metabolic pathway, adhesion and connection metabolism pathway, and nitrogen metabolism pathway. Conclusion There are different serum protein profiles between acute SCI patients and healthy controls. Candidate proteins fructose-bisphosphate aldolase and carbonic anhydrase are promising biomarkers.