成纤维细胞生长因子-7在除草醚诱导的先天性膈疝大鼠模型胎肺中的表达

目的研究成纤维细胞生长因子-7（FGF-7）在先天性膈疝（CDH）胎肺中的表达及探讨其在CDH肺发育不良中的作用。方法采用免疫组织化学和图像分析方法半定量地检测FGF-7在胎肺中的表达部位及表达相对含量。结果CDH肺发育不良，处于假腺体期。FGF-7在对照组显示强表达于支气管和细支气管上皮细胞，在除草醚（nitrofen）组中表达微弱，其表达的相对含量显著低于对照组，Nitrofen组中CDH组和noCDH组间差异没有显著性意义。FGF-7的表达与肺泡面积呈显著正相关性，与肺泡间隔呈显著负相关性。结论FGF-7在CDH胎肺中表达降低，且降低具有组织特异性；FGF-7可作为提示CDH胎肺成熟度的重要指标；FGF-7表达降低可能是CDH肺发育不良形成机制之一。

The expression of fibroblast growth factor-7 in the fetal lungs of nitrofen-induced diaphragmatic hernia in rats

Objective To investigate the expression of fibroblast growth factor-7 (FGF-7) in fe- tal lungs of rats with congenital diaphragmatic hernia (CDH) induced by nitrofen,and to evaluate its role in the pathogenesis of the pulmonary hypoplasia.Methods The CDH in rats was induced by gas- tric injection of nitrofen.The histological changes of the lungs in CDH and control group were visual- ized by routine HE staining.And the expression and contents of FGF-7 in the lungs in these groups were analyzed by immunohistochemistrical staining and image analysis.Results The lungs of CDH fe tuses showed marked hypoplasia and pseudoglandular change.In control lungs,the expression of FGF-7 was strongly noted at bronchial and bronchiolar epithelium.However,in nitrofen experimental group,its expression was weak.Relative contents of FGF-7 significantly decreased in nitrofen experi mental group compared with those in the control group.There was no statistical difference between two subgroups of the nitrofen experimental group.The expression of FGF 7 has positive correlation with the alveolar area and negative correlation with the alveolar interval area.Conclusions The de- creased expression of FGF-7 in CDH fetal lungs has a tissue specialty.FGF-7 can be seen as a impor tant index of maturity in CDH fetal lungs.The decreased expression of FGF-7 probably is an impor- tant effective factor in the pathogenesis of the pulmonary hypoplasia of CDH.