心脏、心神经交感传入神经元的节段性分布及心神经向中枢的投射(HRP法)

用HRP法对心脏交感传入神经元的节段性分布进行了探讨。结果证明,心脏交感传入神经元的节段分布为颈_(8~-)胸_(10);心神经的传入神经元的节段分布为颈_8至胸_9。用 CB-HRP10微升注入10只猫心中及心下神经干后,心神经传入纤维进入脊髓的节段为胸_(1-5),同时在胸_(1-5)脊髓的Ⅲ-ⅤⅡ板层见到被 HRP 标记的纤维,并与Ⅲ、Ⅳ、Ⅴ、ⅤⅡ板层相联系。上述结果为阐明心脏牵涉性痛及针刺治疗心脏疾患(心动过速,心绞痛等症)的机理提供了形态学基础。

The Segmental Distribution of Sympathetic Afferent Neurons of the Heart,Cardiac Nerve,and Projection Of the Cardiac Nerve to the Central Nervous System

1.The HRP labeled method was used to study the segmental distribution of sympathetic afferentneurons of the heart.10%-20% HRP 150ul solution was injected around the left coronary artery of18 cats.The HRP labeled cells were observed in the C_8-T_(10)spinal ganglions,therefore the segmentaldistribution of sympathetic afferent neurons of the heart were in C_8-T_(10)spinal cord.2.The segmental distribution of cardiac nerve's afferent neurons was studied,30% HRP 15μlwas injected into the cardiac nerve of 18 cats.The HRP labeled cells were observed in the C_8-T_9 spinalganglions.3.CB-HRP 10μl was injected into the medium and inferior cardiac nerves.The cardiac nerve'sfibers project into T_(1-5)spinal cord,the HRP labeled fibers were observed in Ⅲ-Ⅶ lamina of spinalcord,and there were connections with Ⅲ,Ⅳ,Ⅴ,Ⅵ,Ⅶ laminas.4.The segmental distribution of sympathetic afferent neurons of heart and cardiac nerve,projec-tion of the cardiac nerve the Ⅲ-Ⅶ laminae of spinal cord and the connection of fibers in the Ⅲ-Ⅶlaminae were the morphologic foundations of referred pain and the heart disease.(tachycordia,anginaetc.)treated with acupuncture.