头孢吡肟、头孢哌酮/舒巴坦、亚胺培南对铜绿假单胞菌体外抗菌活性

目的了解铜绿假单胞菌的血清分型及头孢吡肟、头孢哌酮/舒巴坦、亚胺培南对铜绿假单胞菌体外抗菌活性。方法常规分离培养细菌,获纯培养后用VITEK微生物全自动分析仪或API系统鉴定到种;血清分型按试剂盒操作说明进行;药敏实验采用K-B纸片扩散法和肉汤定量稀释法,按NCCLS规定的标准进行;ESBLs检测采用双纸片确认法,AmpC酶检测采用三维实验确认法。结果100株铜绿假单胞菌分型率为83%,B型占50%,G型和E型分别为9%和6%;产ESBLs和产AmpC的阳性率分别为37%、15%;对常用抗菌药物的耐药率分别为左氧氟沙星51%、阿米卡星48%、亚胺培南38%、头孢吡肟33%、头孢他啶20%、头孢哌酮/舒巴坦18%,头孢吡肟、头孢哌酮/舒巴坦、亚胺培南对铜绿假单胞菌的抑菌率分别为59%、43%、15%。结论铜绿假单胞菌的血清型主要以B型为主,次为G型和E型;该菌产酶率和耐药率已相当严重,临床应根据药敏试验情况合理选择使用抗菌药物。

Study of in vitro antibacterial activity of cefepime,cefoperazone/subactam, imipenem against Pseudomonas aeruginosa

Objective To investigate the serum typing and in vitro antibacterial activity of cefepime,imipenem,cefoperazone/subactam,against Pseudomonas aerugino sa (PSA).Methods All isolates were identified by Vitek microbe automatic instrum ent and API identifying system.PSA were classified by typing serum according to reagent kit specification.Drug sensitive tests used K-B paper disk diffusion an d broth quantitative dilution method in accordnace with NCCLS standards.ESBLs wa s detected by double disk synergy test.AmpC was detected by three-dimensional test.Results The classified rates of 100 PSA isolates were 83%;B type was 50%; G and E type were 9%and 6%respectively.The positive rates of ESBLs and AmpC we re 37%and 15%.The drug resistance rates of levofloxacin,amikacin, imipenem, ce fepime, ceftazidime, cefoperazone/subactam were 51%, 48%, 38%, 33%, 20%and 18%respectively.The inhibition rates of cefepime,cefoperazone/subactam and imip enem against PSA were 59%,43%and 15%respectively. Conclusion The main serum t ypies of PSA were B,G and E type.The zymogenic rates of PSA are very serious. Ra tional selection of antimicrobial agents should be initiated in accordance with results of drug sensitive tests.