| 薄层层析测定胆汁中胆固醇 |
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| 引用本文： | 王云海,姚秉礼,陈坚,李伟.薄层层析测定胆汁中胆固醇[J].新疆医科大学学报,1990(1). |
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| 作者姓名： | 王云海 姚秉礼 陈坚 李伟 |
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| 作者单位： | 新疆医学院第一附属医院普外科，新疆医学院第一附属医院普外科，新疆医学院药学系分析化学教研室，新疆医学院药学系分析化学教研室 |
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| 摘 要： | 本文介绍一种薄层分离-斑点洗脱-比色测定胆汁中胆固醇的方法。玻板铺硅胶G;展开剂异辛烷:醋酸乙酯为2:1(V/V)。胆固醇Rf值0.52,展开后取下胆固醇斑点,比色测定。本法样品用量少,准确度高。
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| 关 键 词： | 薄层色谱 胆固醇 胆汁 |
Determination of Cholesterol in Bile by Thin-Layer Chromatography |
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| Wang Yunhai,et al.Determination of Cholesterol in Bile by Thin-Layer Chromatography[J].Journal of Xinjiang Medical University,1990(1). |
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| Authors: | Wang Yunhai |
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| Institution: | Wang Yunhai,et al Department of Surgery,First Affiliated Hospital,Xinjiangg Medica College |
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| Abstract: | A method of thin-layer chromatolraphy for analyzing cholesterol in bile is reported. The plates are prepared from Kieselgel G.developed solvent system: Isooctane 2 parts, Ethy l acetate 1 part (by volume) . After separation cholesterol spot Rf value is 0.52. This method is exact and practical. |
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| Keywords: | thin-layer chromatography cholesterol bile |
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