马来丝虫幼虫经细胞毒作用后氨基酸的变化

目的观察马来丝虫微丝蚴(mf)经细胞毒作用后氨基酸含量的变化,进一步探讨宿主体内杀虫机制.方法应用高速氨基酸分析仪对细胞毒作用前后的周期型马来丝虫mf进行氨基酸组成及含量的分析比较.结果马来丝虫mf含17种氨基酸,缺少色氨酸.经宿主细胞毒作用后的虫体氨基酸绝对含量明显低于细胞毒作用前(P＜0.05).其中,精氨酸、脯氨酸、甲硫氨酸和赖氨酸下降显著(P＜0.01).结论氨基酸含量下降,尤其是碱性氨基酸与酸性氨基酸、芳香族氨基酸之间比率下降可能是宿主体内杀伤丝虫mf的后果之一.

Studies on amino acids in microfilariae of periodic Brugia malayi before and after cytotoxicity

Objective To study the mechanism of killing microfilariae in host by detecting the change of amino acids in Brugia malayi microfilariae. Methods The composition and contents of amino acid in microfilariae of periodic Brugia malayi before and after the effect of cytotoxicity were analyzed with automatic amino acid analyzer. Results The result showed that microfilariae contained 17 kinds of amino acids, but lacked Tryptophan. The total amount of amino acids of the microfilariae after cytotoxicity was lower than that before cytotoxicity( P <0.05)and there were 4 kinds of amino acids(Arg, Pro, Met, Lys) with the significant difference( P <0.01). The ratio of aliphatic amino acid to acidic as well as aromatic amino acid was markedly lower. Conclusion These results suggest that the decrease of the amino acid in microfilariae, especially the ratio of aliphatic amino acid to acidic as well as aromatic amino acid may be involved in killing microfilariae in host.