Preconceptional paternal glycidamide exposure affects embryonic gene expression: single embryo gene expression study following in vitro fertilization |
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| Authors: | Brevik Asgeir Rusnakova Vendula Duale Nur Slagsvold Hege Holte Olsen Ann-Karin Storeng Ritsa Kubista Mikael Brunborg Gunnar Lindeman Birgitte |
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| Affiliation: | aNorwegian Institute of Public Health, Division of Environmental Medicine, Department of Chemical Toxicology, P.O. Box 4404, Nydalen, N-0403 Oslo, Norway;bDept. of Gene Expression, Institute of Biotechnology AS CR, v.v.i., Videnska 1083, Prague 4, 142 20, Czech Republic;cOslo University Hospital - Rikshospitalet, 0027 Oslo, Norway;dTataa Biocenter, Odinsgatan 28, 411 03 Göteborg, Sweden |
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| Abstract: | Recognition of early determinants of disease onset has sparked an interest in paternally transmitted factors and their impact on the developing embryo. Acrylamide (AA), a widely distributed xenobiotic compound, is converted to its active metabolite glycidamide (GA) by the CYP2E1 enzyme. Based on its capacity to induce dominant lethal mutations, we hypothesized that paternal GA exposure would have a negative impact on embryonic genome activation, via GA-DNA and protamine adducts persisting in the fertilizing sperm. Using a combination of in vitro fertilization (IVF) techniques and RT-qPCR single embryo gene expression (SEGE), we studied the expression of key DNA repair genes and genes important for embryo development, at the 1-, 2-, 4- and 8-cell stage of the developing mouse embryo. Compared to controls paternal GA-exposure gave rise to an altered pattern of embryonic gene expression, with an initial reduced expression at early stages followed by increased expression at the 8-cell stage. |
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| Keywords: | Single-cell gene expression Glycidamide Acrylamide Mouse embryo |
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