| 直接还原法标记^99mTc与抗人膀胱癌单克隆抗体BDI—1 |
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| 引用本文： | 杨德林,周起敬.直接还原法标记^99mTc与抗人膀胱癌单克隆抗体BDI—1[J].昆明医学院学报,1999,20(2):28-30. |
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| 作者姓名： | 杨德林 周起敬 |
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| 作者单位： | 昆明医学院第二附属医院泌尿外科(杨德林，徐鸿毅，石永福，李泽惠)，昆明医学院第二附属医院!昆明650101(周起敬) |
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| 基金项目： | 云南省应用基础研究（ 青年基金） 资助 |
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| 摘 要： | 通过２ － 巯基乙醇直接还原法将抗人膀胱癌单克隆抗体 Ｂ Ｄ Ｉ－ １ 与核素９９ｍ Ｔｃ 进行标记， 制备９９ ｍ Ｔｃ － Ｂ Ｄ Ｉ－ １ ． 优化条件后进行标记并进行一系列检测． 结果： 标记率为７２ ％ ， 经 Ｓｅｐｈａｄｅｘ 柱纯化后标记抗体的放化纯度高于９５ ％ ； 标记抗体的免疫活性分数为８７ ％ ， 与 Ｅ－ Ｊ细胞的结合率为９０ ％ ； 无毒、无菌、无热原． 结果表明： ２ － 巯基乙醇直接还原法将抗人膀胱癌单克隆抗体 Ｂ Ｄ Ｉ－ １ 与核素９９ ｍ Ｔｃ 进行标记为进一步进行膀胱癌的放射免疫显像诊断奠定了基础．
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| 关 键 词： | 锝-99m 单克隆抗体 膀胱肿瘤 放射免疫检测 |
Reduction-Mediated Technetium-99m Labeling of Antihuman Bladder Carcinoma Monoclonal Antibodies BDI-1 |
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| Yang Delin,Xu Hongyi,Zhou Qijing,Shi Yongfu,Li Zehui.Reduction-Mediated Technetium-99m Labeling of Antihuman Bladder Carcinoma Monoclonal Antibodies BDI-1[J].Journal of Kunming Medical College,1999,20(2):28-30. |
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| Authors: | Yang Delin Xu Hongyi Zhou Qijing Shi Yongfu Li Zehui |
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| Institution: | Yang Delin 1) Xu Hongyi 1) Zhou Qijing 2) Shi Yongfu 1) Li Zehui 1) |
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| Abstract: | Anti-human bladder carcinoma cell line BIU-87 monoclonal antibody BDI-1 was labeled with 99mTc by direct mercaptoethanol reducing method. Quality control tests showed that the labeling rate was 72% and the radiochemical purity was higher than 95%; the immunoreactive fraction of 99mTc-BDI-1 was 87% and the binding rate with E-J cell line was 90%. There was no toxity, no apyrogenicity and no bacteria growth in culture. The direct labeling method applied to the preparation of 99mTc labeled antibody BDI-1 was successful. 99mTc-BDI-1 is worthy of further clinical investigation for bladder tumor radioimaging. |
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| Keywords: | Techentium-99m Monoclonal antibodies Bladder neoplasm |
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