| 超氧化物歧化酶两种测活方法的比较 |
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| 引用本文： | 顾含真,陆领倩,袁勤生,赵健,韩瑞贞,周永祥.超氧化物歧化酶两种测活方法的比较[J].药物生物技术,2006,13(5):377-379. |
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| 作者姓名： | 顾含真 陆领倩 袁勤生 赵健 韩瑞贞 周永祥 |
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| 作者单位： | 1. 华东理工大学生物反应器国家重点实验室,上海,200237
2. 苏州新奥生物制品有限公司,江苏,苏州,215111 |
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| 摘 要： | 超氧化物歧化酶（SOD）的测活方法很多，活性单位也不统一，该文研究了改进的肾上腺素法和微量连苯三酚法两种测活方法，比较了两者的自氧化曲线、重现性、灵敏度及测活结果。在严格控制实验条件的情况下，对两种方法的自氧化速率进行了相对标准偏差（RSD％）、线性回归（R）等统计分析。连苯三酚法在重现性（RSD4.72％，n=10）和灵敏度方面均高于肾上腺素法（RSD7.71％，n=10），连苯三酚法与肾上腺素法测活结果的比值约为2．21。
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| 关 键 词： | 超氧化物歧化酶 活性 肾上腺素 连苯三酚 |
| 文章编号： | 1005-8915（2006）05-0377-03 |
| 收稿时间： | 2006-02-19 |
| 修稿时间： | 2006-02-192006-06-04 |
A Comparison between Two Methods for Determination of SOD Activity |
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| GU Han-zhen,LU Ling-qian,YUAN Qin-sheng,ZHAO Jian,HAN Rui-zhen,ZHOU Yong-xiang.A Comparison between Two Methods for Determination of SOD Activity[J].Pharmaceutical Biotechnology,2006,13(5):377-379. |
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| Authors: | GU Han-zhen LU Ling-qian YUAN Qin-sheng ZHAO Jian HAN Rui-zhen ZHOU Yong-xiang |
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| Institution: | 1. State Key Laboratory of Bioreactor Engineering ,East China University of Science and Technology, Shanghai 200237, China ; 2. Suzhou XIN-AO Bio-product Company ,Ltd , Suzhou 215111, China |
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| Abstract: | There are a variety of methods for determination of SOD activity,with different units.Two different methods: epinephrine autoxidation and pyrogallol autoxidation have been studied and incprroved in this paper.We have also made the comparison on the aspect of autoxidative curve,recurring,sensitivity and their respective(results) on SOD activity.Statistics data are made concerning Relative Standardized Deviation(RSD) and Linear Regression(R).The method of pyrogallol autoxidation(RSD 4.72%) is better than that of epinephrine autoxidation(RSD 7.71%) in the above aspects.The ratio of the result of pyrogallol autoxidation and epinephrine autoxidation for determination of SOD activity is about 2.21. |
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| Keywords: | Superoxide Dismutase Activity Epinephrine Pyrogallol |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
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