他莫昔芬对兔耳增生性瘢痕作用的实验研究

目的探讨他莫昔芬对兔耳增生性瘢痕的作用。方法建立兔耳增生性瘢痕模型,将24只兔随机分为高、中、低3个浓度组和1个对照组,分别注射他莫昔芬(140μmol/L、70μmol/L、35μmol/L)和生理盐水。HE及天狼星红染色,计算成纤维细胞密度和Ⅰ、Ⅲ型胶原的比例。结果高、中、低3组成纤维细胞密度均低于对照组,并有显著差异。高浓度组和中浓度组Ⅰ、Ⅲ型胶原比例低于对照组,且有显著差异。结论局部注射他莫昔芬可抑制增生性瘢痕成纤维细胞增殖和胶原合成。

The Effect of Tamoxifen on the Hypertrophic Scar in Rabbit Ear

Objective To study the effect of tamoxifen on the hypertrophic scar in the rabbit ear.Methods Established the acute model of dermal hypertrophic scar in the rabbit ear,24 rabbits were randomly divided into 4 groups.Three groups were treated by 3 kinds of dilutions of tamoxifen,another group was treated saline as comparison.HE-stained and sirius red-stained sections were performed.The numerical density on area of the fibroblast and the proportions of collagen Ⅰ and collagen Ⅲ were calculated.Result Compared with the saline-treated scars,the numerical density on area of the fibroblast in three Tamoxifen-treated groups decreased significantly.The ratio of collagen Ⅰ and collagen Ⅲ decreased significantly.Conclusion Tamoxifen could reduce the fibroblast proliferation and collagen content in the acute model of dermal hypertrophic scar in the rabbit ear.